Nitric oxide responses in Arabidopsis hypocotyls are mediated by diverse phytohormone pathways
| Autor: | Alberto Coego, Álvaro Costa-Broseta, José León, Mari-Cruz Castillo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis thaliana Physiology Arabidopsis Plant Science 01 natural sciences Hypocotyl Transcriptome Ethylene 03 medical and health sciences chemistry.chemical_compound Plant Growth Regulators Gene Expression Regulation Plant Etiolation Brassinosteroids Transcription factors Brassinosteroid Transcription factor Abscisic acid Plant Proteins Strigolactones Regulation of gene expression Salicylate biology fungi food and beverages Nitric oxide biology.organism_classification Research Papers Cell biology 030104 developmental biology ABA chemistry Seedlings Plant—Environment Interactions TRANSPLANTA lines 010606 plant biology & botany |
| Zdroj: | Journal of Experimental Botany RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia instname |
| ISSN: | 1460-2431 0022-0957 |
| DOI: | 10.1093/jxb/ery286 |
| Popis: | An NO-sensing mechanism controlling hypocotyl growth in etiolated seedlings requires the biosynthesis and/or signaling of ethylene, strigolactones, salicylate, abscisic acid, and brassinosteroids. Plants are often exposed to high levels of nitric oxide (NO) that affects development and stress-triggered responses. However, the way in which plants sense NO is still largely unknown. Here we combine the analysis of early changes in the transcriptome of plants exposed to a short acute pulse of exogenous NO with the identification of transcription factors (TFs) involved in NO sensing. The NO-responsive transcriptome was enriched in hormone homeostasis- and signaling-related genes. To assess events involved in NO sensing in hypocotyls, we used a functional sensing assay based on the NO-induced inhibition of hypocotyl elongation in etiolated seedlings. Hormone-related mutants and the TRANSPLANTA collection of transgenic lines conditionally expressing Arabidopsis TFs were screened for NO-triggered hypocotyl shortening. These approaches allowed the identification of hormone-related TFs, ethylene perception and signaling, strigolactone biosynthesis and signaling, and salicylate production and accumulation that are essential for or modulate hypocotyl NO sensing. Moreover, NO inhibits hypocotyl elongation through the positive and negative regulation of some abscisic acid (ABA) receptors and transcripts encoding brassinosteroid signaling components thereby also implicating these hormones in NO sensing. |
| Databáze: | OpenAIRE |
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