Plasminogen activator, tissue type regulates germinal vesicle breakdown and cumulus expansion of bovine cumulus-oocyte complex in vitro†
| Autor: | Gerile Subudeng, Zhihong Liu, Erge Namei, Bingxue Yang, Boyang Yu, Yue Bai, Chen-Guang Du, Yufen Zhao, Haijun Li |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Adenosine monophosphate Biology Tissue plasminogen activator 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Oogenesis Gene expression Plasminogen Activator Inhibitor 1 medicine Animals Cells Cultured Cell Proliferation Messenger RNA 030219 obstetrics & reproductive medicine Germinal vesicle Cumulus Cells urogenital system Cell Biology General Medicine Oocyte In vitro maturation Cell biology In Vitro Oocyte Maturation Techniques 030104 developmental biology medicine.anatomical_structure Reproductive Medicine chemistry Tissue Plasminogen Activator Oocytes Cattle Female Transcriptome Plasminogen activator medicine.drug |
| Zdroj: | Biology of reproduction. 100(6) |
| ISSN: | 1529-7268 |
| Popis: | Plasminogen activator, tissue type (PLAT) and its inhibitor serpin family E member 1 (SERPINE1) cooperatively regulate PLAT activity in various reproductive processes. However, it is unknown whether this includes bovine oocyte maturation. We addressed this question in the present study by evaluating PLAT and SERPINE1 protein localization in immature cumulus-oocyte complexes (COCs), as well as PLAT mRNA and protein expression in cultured COCs after 0, 8, 16, and 24 h of in vitro maturation (IVM). We also examined the effects of PLAT and SERPINE1 on germinal vesicle breakdown (GVBD) and oocyte cyclic 3' 5' adenosine monophosphate (cAMP) levels, cumulus expansion index, and expansion-related gene expression in oocytes derived from bovine COCs cultured for 4, 8, and 12 h and in COCs cultured for 16 h. Both PLAT and SERPINE1 localized in cumulus cells but only the latter was detected in oocytes. PLAT and SERPINE1 transcript levels increased during IVM; however, from 8 to 16 h, the levels of PLAT remained stable whereas those of SERPINE1 increased, resulting in a decline in PLAT concentration. Additionally, PLAT delayed GVBD, increased oocyte cAMP levels, and blocked cumulus expansion and associated gene expression, which was reversed by SERPINE1 supplemented. Thus, PLAT delays bovine oocyte GVBD by enhancing oocyte cAMP levels during the first 8 h of IVM; suppression of PLAT activity via accumulation of SERPINE1 in COCs results in cumulus expansion from 8 to 16 h of IVM. These findings provide novel insights into the molecular mechanisms underlying in vitro bovine oocyte maturation. |
| Databáze: | OpenAIRE |
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