Synaptophysin Is a Reliable Marker for Axonal Damage
| Autor: | Markus Kipp, Kurt-Wolfram Sühs, Sandra Amor, Martin Stangel, Wolfgang Baumgärtner, Andreas Beineke, Lijie Gai, Viktoria Gudi, Vanessa Herder, Florian Hansmann, Thomas Skripuletz, Laura Salinas Tejedor |
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| Přispěvatelé: | Amsterdam Neuroscience - Neuroinfection & -inflammation, Pathology |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Corpus callosum Pathology and Forensic Medicine White matter 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine mental disorders medicine Amyloid precursor protein Remyelination Microglia biology Multiple sclerosis General Medicine medicine.disease 030104 developmental biology medicine.anatomical_structure Neurology nervous system Synaptophysin biology.protein Immunohistochemistry Neurology (clinical) 030217 neurology & neurosurgery |
| Zdroj: | Gudi, V, Gai, L, Herder, V, Tejedor, L S, Kipp, M, Amor, S, Sühs, K-W, Hansmann, F, Beineke, A, Baumgärtner, W, Stangel, M & Skripuletz, T 2017, ' Synaptophysin Is a Reliable Marker for Axonal Damage ', Journal of Neuropathology and Experimental Neurology, vol. 76, no. 2, pp. 109-125 . https://doi.org/10.1093/jnen/nlw114 Journal of Neuropathology and Experimental Neurology, 76(2), 109-125. Lippincott Williams and Wilkins |
| ISSN: | 0022-3069 |
| DOI: | 10.1093/jnen/nlw114 |
| Popis: | Synaptophysin is an abundant membrane protein of synaptic vesicles. The objective of this study was to determine the utility of identifying synaptophysin accumulations (spheroids/ovoids/bulbs) in CNS white matter as an immunohistochemical marker of axonal damage in demyelinating and neuroinflammatory conditions. We studied the cuprizone toxicity and Theiler’s murine encephalomyelitis virus (TMEV) infection models of demyelination and analyzed CNS tissue from patients with multiple sclerosis (MS). Synaptophysin colocalized with the amyloid precursor protein (APP), a well-known marker of axonal damage. In the cuprizone model, numerous pathological synaptophysin/APP-positive spheroids/ovoids were identified in the corpus callosum at the onset of demyelination; the extent of synaptophysin/APP-positive vesicle aggregates correlated with identified reactive microglia; during late and chronic demyelination, the majority of synaptophysin/APP-positive spheroids/ovoids resolved but a few remained, indicating persistent axonal damage; in the remyelination phase, scattered large synaptophysin/APP-positive bulbs persisted. In the TMEV model, only a few large- to medium-sized synaptophysin/APP-positive bulbs were found in demyelinated areas. In MS patient tissue samples, the bulbs appeared exclusively at the inflammatory edges of lesions. In conclusion, our data suggest that synaptophysin as a reliable marker of axonal damage in the CNS in inflammatory/demyelinating conditions. |
| Databáze: | OpenAIRE |
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