Screening and Molecular Identification of Novel Pectinolytic Bacteria from Forest Soil
| Autor: | Aristide Laurel Mokale Kognou, Sihai Han, Xiaodong Zhang, Sarita Shrestha, Xuatong Chen, Feifei Chen, Wensheng Qin, Chonlong Chio, Janak Raj Khatiwada |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
0106 biological sciences
food.ingredient Pectin Plant Science Cellulase 01 natural sciences Biochemistry Genetics and Molecular Biology (miscellaneous) Streptomyces soil 03 medical and health sciences Hydrolysis food 010608 biotechnology pectinase enzyme screening identification oil extraction juice extraction Food science Pectinase 030304 developmental biology 2. Zero hunger lcsh:TP500-660 0303 health sciences biology Chemistry lcsh:Fermentation industries. Beverages. Alcohol biology.organism_classification 16S ribosomal RNA 6. Clean water Xylanase biology.protein Bacteria Food Science |
| Zdroj: | Fermentation; Volume 7; Issue 1; Pages: 40 Fermentation, Vol 7, Iss 40, p 40 (2021) |
| ISSN: | 2311-5637 |
| DOI: | 10.3390/fermentation7010040 |
| Popis: | Pectinases are a group of enzymes with broad application, including in plant fiber processing, pectic wastewater treatment, paper pulping, fruit juice extraction, and clarification. With an increasing industrial demand for these enzymes, it is useful to isolate organisms that produce large amounts of pectinase and possess wide ranges of stability factors like temperature and pH. In this study, 17 out of 29 bacteria (58.62%) from forest soil samples were pectinolytic. However, only four bacteria (S-5, S-10, S-14, and S-17) showed high pectin hydrolysis zones (ranging from 0.2 cm to 1.7 cm). These four bacteria were identified based on colony morphology, microscopic characterization, biochemical characteristics, and 16S rDNA sequencing. They were designated as Streptomyces sp. (S-5, S-14), Cellulomonas sp. (S-10), and Bacillus sp. (S-17). Interestingly, bacteria showed cellulase and xylanase activity in addition to pectinase. The quantitative assay for pectinase activity of the four isolates provided proof that they are pectinase producers and can be considered potential candidates for industrial uses. The crude enzyme extracts of these bacteria are applicable in oil and juice extraction from sesame seeds and apples, respectively. |
| Databáze: | OpenAIRE |
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