Impaired Junctions and Invaded Macrophages in Oral Epithelia With Oral Pain
| Autor: | Atsushi Danjo, Tomoko Kitsuki, Reiko U. Yoshimoto, Reona Aijima, Yasuyoshi Ohsaki, Yukiko Ohyama, Junko Yoshizumi, Ai Lin Cao, Tamotsu Kiyoshima, Mizuho A. Kido, Yoshio Yamashita |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Histology Adhesion (medicine) Pain TRPV Cation Channels Filamentous actin Adherens junction 03 medical and health sciences Ballooning degeneration Mice Young Adult Edema medicine Cell Adhesion Animals Humans Labial Mucosa beta Catenin Aged 0303 health sciences Mouth Cell adhesion molecule business.industry Macrophages 030302 biochemistry & molecular biology Epithelial Cells Articles Middle Aged medicine.disease Cadherins Immunohistochemistry Actins Female Anatomy medicine.symptom business Immunostaining |
| Popis: | Recurrent or chronic oral pain is a great burden for patients. Recently, the links between epithelial barrier loss and disease were extended to include initiation and propagation. To explore the effects of pathohistological changes in oral epithelia on pain, we utilized labial mucosa samples in diagnostic labial gland biopsies from patients with suspected Sjögren’s syndrome (SS), because they frequently experience pain and discomfort. In most labial mucosa samples from patients diagnosed with SS, disseminated epithelial cellular edema was prevalent as ballooning degeneration. The disrupted epithelia contained larger numbers of infiltrating macrophages in patients with oral pain than in patients without pain. Immunohistochemistry revealed that edematous areas were distinct from normal areas, with disarranged cell–cell adhesion molecules (filamentous actin, E-cadherin, β-catenin). Furthermore, edematous areas were devoid of immunostaining for transient receptor potential channel vanilloid 4 (TRPV4), a key molecule in adherens junctions. In an investigation on whether impaired TRPV4 affect cell–cell adhesion, calcium stimulation induced intimate cell–cell contacts among oral epithelial cells from wild-type mice, while intercellular spaces were apparent in cells from TRPV4-knockout mice. The present findings highlight the relationship between macrophages and epithelia in oral pain processing, and identify TRPV4-mediated cell–cell contacts as a possible target for pain treatment. |
| Databáze: | OpenAIRE |
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