Morphological and Transcriptional Changes in Human Bone Marrow During Natural Plasmodium vivax Malaria Infections
| Autor: | Lauro Sumoy, Bàrbara Baro, Alberto Ayllon-Hermida, Carmen Fernandez-Becerra, Marcelo A M Brito, Wuelton Marcelo Monteiro, Izabella Picinin Safe, Erich Vinicius De Paula, Katrien Deroost, Erick F. G. Figueiredo, Maria P. Armengol, Marcus V. G. Lacerda, Hernando A. del Portillo, Anne Cristine Gomes de Almeida, Allyson Guimarães Costa, Bidossessi Wilfried Hounkpe, Tainá Raiol |
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| Rok vydání: | 2022 |
| Předmět: |
0301 basic medicine
Ineffective erythropoiesis 030231 tropical medicine Plasmodium vivax Cell Malària Transferrin receptor Biology medicine.disease_cause Transcriptome natural infections 03 medical and health sciences 0302 clinical medicine Bone Marrow medicine Malaria Vivax Immunology and Allergy Animals Humans Bone marrow Erythropoiesis Gene ineffective erythropoiesis RNA Anemia RNA sequencing biology.organism_classification Malaria 030104 developmental biology Infectious Diseases medicine.anatomical_structure Medul·la òssia Immunology bone marrow aspirates |
| Zdroj: | Journal of Infectious Diseases r-IGTP. Repositorio Institucional de Producción Científica del Instituto de Investigación Germans Trias i Pujol instname Dipòsit Digital de la UB Universidad de Barcelona |
| ISSN: | 1537-6613 |
| Popis: | - Label: BACKGROUND NlmCategory: BACKGROUND content: The presence of Plasmodium vivax malaria parasites in the human bone marrow (BM) is still controversial. However, recent data from a clinical case and experimental infections in splenectomized nonhuman primates unequivocally demonstrated the presence of parasites in this tissue. - Label: METHODS NlmCategory: METHODS content: In the current study, we analyzed BM aspirates of 7 patients during the acute attack and 42 days after drug treatment. RNA extracted from CD71+ cell suspensions was used for sequencing and transcriptomic analysis. - Label: RESULTS NlmCategory: RESULTS content: We demonstrated the presence of parasites in all patients during acute infections. To provide further insights, we purified CD71+ BM cells and demonstrated dyserythropoiesis and inefficient erythropoiesis in all patients. In addition, RNA sequencing from 3 patients showed that genes related to erythroid maturation were down-regulated during acute infections, whereas immune response genes were up-regulated. - Label: CONCLUSIONS NlmCategory: CONCLUSIONS content: This study thus shows that during P. vivax infections, parasites are always present in the BM and that such infections induced dyserythropoiesis and ineffective erythropoiesis. Moreover, infections induce transcriptional changes associated with such altered erythropoietic response, thus highlighting the importance of this hidden niche during natural infections. |
| Databáze: | OpenAIRE |
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