Genetic and functional analyses of krs, a locus encoding kurstakin, a lipopeptide produced by Bacillus thuringiensis

Autor: Thibault Caradec, Leyla Slamti, Didier Lereclus, Alexis Canette, Michel Gohar, Myriam Gominet, Sébastien Gélis-Jeanvoine, Christelle Lemy, Philippe Jacques
Přispěvatelé: MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, Laboratoire de Procédés Biologiques, Génie Enzymatique et Microbien (ProBioGEM), Université de Lille, Sciences et Technologies-École polytechnique universitaire de Lille (Polytech Lille), Biologie des Bactéries pathogènes à Gram-positif, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), This study was funded by the French 'Agence Nationale de la Recherche' (Bt-Surf, ANR-12-EMMA-0005). S. Gélis-Jeanvoine was supported by a fellowship from the French 'Ministère de l'enseignement supérieur et de la recherche'., ANR-12-EMMA-0005,Bt-Surf,Caractérisation et production de biosurfactants de Bacillus thuringiensis(2012), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2017
Předmět:
0301 basic medicine
MESH: Operon
Operon
[SDV]Life Sciences [q-bio]
030106 microbiology
Population
Bacillus thuringiensis
Locus (genetics)
MESH: Biofilms
Biology
MESH: Quorum Sensing
Microbiology
Spo0A
Lipopeptides
03 medical and health sciences
chemistry.chemical_compound
Transcriptional regulation
Bacterial Proteins
MESH: Lipopeptides
Transcription (biology)
MESH: Peptide Biosynthesis
Nucleic Acid-Independent
MESH: Promoter Regions
Genetic
Bacillus cereus group
Promoter Regions
Genetic
education
MESH: Bacterial Proteins
Molecular Biology
Gene
Genetics
MESH: Gene Expression Regulation
Bacterial
education.field_of_study
MESH: Bacillus thuringiensis
Biofilm
fungi
Quorum Sensing
Lipopeptide
Promoter
Gene Expression Regulation
Bacterial
General Medicine
biology.organism_classification
chemistry
Biofilms
Peptide Biosynthesis
Nucleic Acid-Independent
Zdroj: Research in Microbiology
Research in Microbiology, Elsevier, 2017, 168 (4), pp.356-368. ⟨10.1016/j.resmic.2016.06.002⟩
Research in Microbiology, 2017, 168 (4), pp.356-368. ⟨10.1016/j.resmic.2016.06.002⟩
ISSN: 0923-2508
DOI: 10.1016/j.resmic.2016.06.002
Popis: Bacteria of the Bacillus genus are able to synthesize several families of lipopeptides. These small molecules are the product of non-ribosomal peptide synthetases. In 2000, it was found that Bacillus thuringiensis, an entomopathogenic bacterium of the Bacillus cereus group, produced a previously unknown lipopeptide: kurstakin. Genomic analyses reveal that the krs locus, encoding the kurstakin synthetases, is specific to the B. cereus group, but is unevenly distributed within this group. Previous work showed that krs transcription requires the necrotrophism quorum-sensor NprR. Here, we demonstrated that the genes of the krs locus form an operon and we defined its transcription start site. Following krs transcription at the population and single-cell levels in multiple culture conditions, we depicted a condition-dependent transcription pattern, indicating that production of kurstakin is subject to environmental regulation. Consistent with this idea, we found krs transcription to be regulated by another master regulator, Spo0A, suggesting that krs expression is fine-tuned by integrating multiple signals. We also reported an unknown DNA palindrome in the krs promoter region that modulates krs expression. Due to their surfactant properties, lipopeptides could play several physiological roles. We showed that the krs locus was required for proper biofilm structuration.
Databáze: OpenAIRE
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