Cellobiose dehydrogenase from the agaricomycete Coprinellus aureogranulatus and its application for the synergistic conversion of rice straw

Autor: Le Xuan Duy, Tran Thi Nhu Hang, Le Mai Huong, Enrico Büttner, Dang Thu Quynh, An Verberckmoes, Christiane Liers, Martin Hofrichter, Do Huu Nghi, Ludo Diels, Harald Kellner, Vu Dinh Giap
Rok vydání: 2021
Předmět:
Zdroj: APPLIED BIOLOGICAL CHEMISTRY
ISSN: 2468-0842
2468-0834
DOI: 10.1186/s13765-021-00637-y
Popis: From the biotechnological viewpoint, the enzymatic disintegration of plant lignocellulosic biomass is a promising goal since it would deliver fermentable sugars for the chemical sector. Cellobiose dehydrogenase (CDH) is a vital component of the extracellular lignocellulose-degrading enzyme system of fungi and has a great potential to improve catalyst efficiency for biomass processing. In the present study, a CDH from a newly isolated strain of the agaricomycete Coprinellus aureogranulatus (CauCDH) was successfully purified with a specific activity of 28.9 U mg−1. This pure enzyme (MW = 109 kDa, pI = 5.4) displayed the high oxidative activity towards β-1–4-linked oligosaccharides. Not least, CauCDH was used for the enzymatic degradation of rice straw without chemical pretreatment. As main metabolites, glucose (up to 165.18 ± 3.19 mg g−1), xylose (64.21 ± 1.22 mg g−1), and gluconic acid (5.17 ± 0.13 mg g−1) could be identified during the synergistic conversion of this raw material with the fungal hydrolases (e.g., esterase, cellulase, and xylanase) and further optimization by using an RSM statistical approach.
Databáze: OpenAIRE
Externí odkaz: