GID1 expression is associated with ovule development of sexual and apomictic plants
Autor: | Diva Maria de Alencar Dusi, Marta Adelina Mendes, Ana Cristina Meneses Mendes Gomes, André Southernman Teixeira Irsigler, Luciana Gomes Ferreira, Vera Tavares de Campos Carneiro, Lucia Colombo |
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Rok vydání: | 2017 |
Předmět: |
0106 biological sciences
0301 basic medicine Arabidopsis Brachiaria Flowers Plant Science Biology Poaceae 01 natural sciences 03 medical and health sciences Gene Expression Regulation Plant Apomixis Botany Arabidopsis thaliana Amino Acid Sequence Ovule Plant Proteins Sequence Homology Amino Acid Arabidopsis Proteins Gene Expression Regulation Developmental food and beverages General Medicine Plants Genetically Modified Megagametogenesis biology.organism_classification Cell biology 030104 developmental biology Pollen Ectopic expression Megaspore mother cell Megaspore Agronomy and Crop Science 010606 plant biology & botany |
Zdroj: | Plant Cell Reports. 37:293-306 |
ISSN: | 1432-203X 0721-7714 |
Popis: | BbrizGID1 is expressed in the nucellus of apomictic Brachiaria brizantha, previous to aposporous initial differentiation. AtGID1a overexpression triggers differentiation of Arabidopsis thaliana MMC-like cells, suggesting its involvement in ovule development. GIBBERELLIN-INSENSITIVE DWARF1 (GID1) is a gibberellin receptor previously identified in plants and associated with reproductive development, including ovule formation. In this work, we characterized the Brachiaria brizantha GID1 gene (BbrizGID1). BbrizGID1 showed up to 92% similarity to GID1-like gibberellin receptors of other plants of the Poaceae family and around 58% to GID1-like gibberellin receptors of Arabidopsis thaliana. BbrizGID1 was more expressed in ovaries at megasporogenesis than in ovaries at megagametogenesis of both sexual and apomictic plants. In ovules, BbrizGID1 transcripts were detected in the megaspore mother cell (MMC) of sexual and apomictic B. brizantha. Only in the apomictic plants, expression was also observed in the surrounding nucellar cells, a region in which aposporous initial cells differentiate to form the aposporic embryo sac. AtGID1a ectopic expression in Arabidopsis determines the formation of MMC-like cells in the nucellus, close to the MMC, that did not own MMC identity. Our results suggest that GID1 might be involved in the proper differentiation of a single MMC during ovule development and provide valuable information on the role of GID1 in sexual and apomictic reproduction. |
Databáze: | OpenAIRE |
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