Effect of glutaraldehyde-fixation on the immunogenicity, particle stability and antigenic reactivity of alfalfa mosaic virus, and the specificity of elicited antibodies
| Autor: | R.I.B. Francki, M.R. Hajimorad |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Antiserum
Antigenicity animal structures Immunogen biology viruses Immunogenicity Antibodies Viral biology.organism_classification Virology Virus Epitopes Fixatives Capsid Alfalfa mosaic virus Antigen Antibody Specificity Glutaral Mosaic Viruses Polyclonal antibodies embryonic structures biology.protein Antigens Viral Medicago sativa |
| Zdroj: | Journal of Virological Methods. 33:13-25 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/0166-0934(91)90003-i |
| Popis: | Glutaraldehyde-fixation was shown to stabilize the structural integrity of alfalfa mosaic virus (AMV) particles as well as to increase their immunogenicity and antigenic reactivity. The antigenic reactivity of the particles was substantially increased irrespective of whether the antibodies were from animals immunized with native or fixed AMV, or preparations of coat protein subunits isolated from the virus. No significant changes in the antigenic specificity of AMV particles were detected following glutaraldehyde-fixation. Thus it is possible to raise antisera to AMV with higher titres by using fixed virus as immunogen. |
| Databáze: | OpenAIRE |
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