Selection and functional analysis of a Pyropia yezoensis ammonium transporter PyAMT1 in potassium deficiency
| Autor: | Eri Adams, Ryoung Shin, Koji Mikami |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Sodium Potassium chemistry.chemical_element Context (language use) Plant Science Aquatic Science Biology medicine.disease_cause 01 natural sciences 03 medical and health sciences Potassium deficiency medicine Salt tolerance Gene Escherichia coli cDNA library Transporter Ammonium transporter Full-length cDNA library 030104 developmental biology Biochemistry chemistry Pyropia yezoensis 010606 plant biology & botany |
| Zdroj: | Journal of Applied Phycology. 29:2617-2626 |
| ISSN: | 1573-5176 0921-8971 |
| Popis: | Seaweeds are believed to have developed unique mechanisms to maintain optimal cellular potassium and sodium concentrations in order to survive in the saline marine environment. To gain a molecular understanding of underlying potassium/sodium homeostasis in seaweeds, full-length cDNA libraries from the multiple stages in the life cycle, including gametophytes, conchosporangia and sporophytes of a marine red alga, Pyropia yezoensis, were constructed. A large portion of genes from each library through the life cycle was revealed to be functionally unknown reconfirming the uniqueness of P. yezoensis genes in terms of evolutionary lineage. Genes that could potentially contribute to potassium deficiency tolerance were selected from the potassium uptake defective Escherichia coli strain expressing gametophytes and conchosporangia libraries under the low potassium conditions. Of those, an ammonium transporter gene, PyAMT1, was demonstrated to enhance potassium deficiency tolerance effectively when expressed in the E. coli strain. Potential roles of PyAMT1 and other candidate components in this context are discussed. |
| Databáze: | OpenAIRE |
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