Oxidant stress enhances Lyso-PAF-AcT activity by modifying phospholipase D and phosphatidic acid in aortic endothelial cells
| Autor: | Yu-Zhang Cao, Lorraine M. Sordillo, C.M. Corl, Z.S. Cohen |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
medicine.medical_specialty
Endothelium Biophysics Phosphatidic Acids medicine.disease_cause Biochemistry Antioxidants Selenium chemistry.chemical_compound Enzyme activator Acetyltransferases Internal medicine Phospholipase D medicine Animals Platelet Activating Factor Molecular Biology Aorta Cells Cultured chemistry.chemical_classification Reactive oxygen species Platelet-activating factor Cell Biology Phosphatidic acid respiratory system Glutathione Enzyme Activation Oxidative Stress Endocrinology medicine.anatomical_structure chemistry Acetyltransferase Cattle lipids (amino acids peptides and proteins) Endothelium Vascular Reactive Oxygen Species Oxidative stress Protein Binding |
| Zdroj: | Biochemical and Biophysical Research Communications. 302:610-614 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/s0006-291x(03)00223-7 |
| Popis: | Oxidant stress, as a consequence of selenium (Se) deficiency, alters production of vasoactive compounds including platelet-activating factor (PAF). Recent studies report that enhanced PAF production during Se deficiency is a consequence of increased lyso-PAF:acetyl-coenzyme A acetyltransferase (Lyso-PAF-AcT) activity. To elucidate the mechanism behind increased Lyso-PAF-AcT activity during oxidant stress, phospholipase D (PLD) activity and phosphatidic acid (PA) production were examined. Increased PLD activity and PA production were exhibited in bovine aortic endothelial cells using a Se-deficient model of oxidant stress. The direct effects of PLD and PA on Lyso-PAF-AcT activity were assessed using selective inhibitors and repletion experiments. Following the inhibition of PLD and addition of exogenous PA, Lyso-PAF-AcT activity significantly decreased and increased, respectively. Therefore, Se deficiency enhances Lyso-PAF-AcT activity in part by modifying PLD and PA. This suggests a novel link between Se status and PAF production, providing potential upstream therapeutic targets for PAF regulation under conditions of oxidant stress. |
| Databáze: | OpenAIRE |
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