Inhibition of transcription and translation in dorsal hippocampus does not interfere with consolidation of memory of intense training
| Autor: | James L. McGaugh, Andrea C. Medina, Gina L. Quirarte, Paola C. Bello-Medina, María E. Torres-García, Luis Miguel Rodríguez-Serrano, Roberto A. Prado-Alcalá |
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| Rok vydání: | 2019 |
| Předmět: |
Male
medicine.medical_specialty Transcription Genetic Cognitive Neuroscience Experimental and Cognitive Psychology Stimulation Hippocampal formation Hippocampus 050105 experimental psychology 03 medical and health sciences Behavioral Neuroscience chemistry.chemical_compound 0302 clinical medicine Internal medicine Gene expression Avoidance Learning medicine Protein biosynthesis Animals 0501 psychology and cognitive sciences Rats Wistar Anisomycin Memory Consolidation Nucleic Acid Synthesis Inhibitors Protein Synthesis Inhibitors Electroshock Protein synthesis inhibitor business.industry 05 social sciences Impaired memory Rats Endocrinology chemistry Protein Biosynthesis Memory consolidation business Neuroscience Dichlororibofuranosylbenzimidazole 030217 neurology & neurosurgery |
| Zdroj: | Neurobiology of Learning and Memory. 166:107092 |
| ISSN: | 1074-7427 |
| DOI: | 10.1016/j.nlm.2019.107092 |
| Popis: | Findings of several experiments indicate that many treatments that typically interfere with memory consolidation are ineffective in preventing or attenuating memory induced by intense training. As extensive evidence suggests that the consolidation of newly acquired memories requires gene expression and de novo protein synthesis the present study investigated whether intense training prevents consolidation impairment induced by blockers of mRNA and protein synthesis. Rats were given a single inhibitory training trial using a moderate (1.0 mA) or a relatively intense (2.0 mA) foot-shock. Bilateral hippocampal infusions of the mRNA synthesis blocker DRB (10, 40 or 80 ng/0.5 µL/hemisphere) or the protein synthesis inhibitor anisomycin (ANI), an inhibitor de novo protein synthesis (15.62, 31.25, or 62.50 µg/0.5 µL/hemisphere) were administered 15 min prior to training. Retention was measured at 30 min or 48 h following training. DRB and ANI impaired memory of moderate training in a dose-dependent manner without affecting short-term memory. In contrast, memory consolidation was not impaired in the groups trained with 2.0 mA. The findings showed that: (1) inhibitors of transcription and translation in the hippocampus impair the consolidation of memory of inhibitory avoidance learning induced by moderate levels of aversive stimulation and (2) blocking of mRNA and protein synthesis does not prevent the consolidation of memory induced by relatively high levels of aversive stimulation. These findings do not support the hypothesis that gene expression and de novo protein synthesis are necessary steps for long-term memory formation as memory was not impaired if intense foot-shock was used in training. |
| Databáze: | OpenAIRE |
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