Glyphosate Determination by Coupling an Immuno-Magnetic Assay with Electrochemical Sensors
| Autor: | Aline Romero Natale, Ilaria Palchetti, Eduardo Torres, Francesca Bettazzi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Calibration curve
Glycine 02 engineering and technology Electrochemistry lcsh:Chemical technology 01 natural sciences Biochemistry Article magnetic beads Analytical Chemistry Electrochemical cell chemistry.chemical_compound electrochemical assay Magnetics glyphosate Limit of Detection lcsh:TP1-1185 Electrical and Electronic Engineering Instrumentation Detection limit Immunoassay Chromatography Herbicides Beer Disposable electrodes Electrochemical assay Glyphosate Magnetic beads Atomic and Molecular Physics and Optics 010401 analytical chemistry Substrate (chemistry) Electrochemical Techniques Contamination 021001 nanoscience & nanotechnology disposable electrodes 0104 chemical sciences chemistry 0210 nano-technology |
| Zdroj: | Sensors (Basel, Switzerland) Sensors Volume 18 Issue 9 Sensors, Vol 18, Iss 9, p 2965 (2018) |
| ISSN: | 1424-8220 |
| Popis: | Glyphosate (N-(phosphonomethyl)glycine) is the most frequently used broad-spectrum herbicide worldwide. Its mechanism of action is based on the inhibition of an enzyme that is essential to plant growth. Its intensive use has caused global contamination to occur, which has not only affected the ecosystems, but even food and other objects of common use. Thus, there is a pronounced need for developing analytical methods for glyphosate determination in different matrices. Here, an electrochemical competitive immunoassay, based on the use of antibody-modified magnetic particles, has been developed. Tetramethylbenzidine (TMB) has been used as an enzymatic substrate. The extent of the affinity reaction has been achieved by monitoring the current value, due to the reduction of the enzymatic product. A disposable screen-printed electrochemical cell has been used. The calibration curve has been recorded in the 0&ndash 10,000 ng/L concentration range, with a detection limit of 5 ng/L and quantification limit of 30 ng/L. The electrochemical immunoassay has also been applied to the analysis of spiked beer samples. |
| Databáze: | OpenAIRE |
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