The Role of Neurotrophic Factors Conjugated to Iron Oxide Nanoparticles in Peripheral Nerve Regeneration:In VitroStudies

Autor: Abraham Shahar, Shlomo Margel, Hadas Skaat, Stefano Geuna, Claudia Grothe, Ofra Ziv-Polat, Itay Levy, Kirsten Haastert-Talini, Federica Fregnan, Sara Neuman
Rok vydání: 2014
Předmět:
medicine.medical_specialty
Time Factors
Article Subject
Basic fibroblast growth factor
lcsh:Medicine
Nerve fiber
Ferric Compounds
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
chemistry.chemical_compound
Myelin
0302 clinical medicine
Dorsal root ganglion
Neurotrophic factors
Ganglia
Spinal
Glial cell line-derived neurotrophic factor
Animals
Medicine
Glial Cell Line-Derived Neurotrophic Factor
Nerve Growth Factors
Peripheral Nerves
Cells
Cultured
Myelin Sheath
030304 developmental biology
0303 health sciences
General Immunology and Microbiology
biology
business.industry
lcsh:R
General Medicine
Nerve Regeneration
Rats
Surgery
Cell biology
Nerve growth factor
medicine.anatomical_structure
nervous system
chemistry
Peripheral nervous system
biology.protein
Nanoparticles
business
030217 neurology & neurosurgery
Research Article
Zdroj: BioMed Research International, Vol 2014 (2014)
BioMed Research International
ISSN: 2314-6141
2314-6133
DOI: 10.1155/2014/267808
Popis: Local delivery of neurotrophic factors is a pillar of neural repair strategies in the peripheral nervous system. The main disadvantage of the free growth factors is their short half-life of few minutes. In order to prolong their activity, we have conjugated to iron oxide nanoparticles three neurotrophic factors: nerve growth factor (βNGF), glial cell-derived neurotrophic factor (GDNF), and basic fibroblast growth factor (FGF-2). Comparative stability studies of free versus conjugated factors revealed that the conjugated neurotrophic factors were significantly more stable in tissue cultures and in medium at 37°C. The biological effects of free versus conjugated neurotrophic factors were examined on organotypic dorsal root ganglion (DRG) cultures performed in NVR-Gel, composed mainly of hyaluronic acid and laminin. Results revealed that the conjugated neurotrophic factors enhanced early nerve fiber sprouting compared to the corresponding free factors. The most meaningful result was that conjugated-GDNF, accelerated the onset and progression of myelin significantly earlier than the free GDNF and the other free and conjugated factors. This is probably due to the beneficial and long-acting effect that the stabilized conjugated-GDNF had on neurons and Schwann cells. These conclusive results make NVR-Gel enriched with conjugated-GDNF, a desirable scaffold for the reconstruction of severed peripheral nerve.
Databáze: OpenAIRE
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