Comparison of Enrichment Procedures for Shiga Toxin–Producing Escherichia coli in Wastes from Commercial Swine Farms
| Autor: | Mark Mogler, Michael A. Grant, D. L. Harris |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Swine
Colony Count Microbial Biology medicine.disease_cause Microbiology Shiga Toxin Food and drug administration Feces chemistry.chemical_compound Shiga-like toxin STX2 Enterotoxigenic Escherichia coli medicine Animals Humans Food science Escherichia coli Shiga toxin-producing Escherichia coli Shiga-Toxigenic Escherichia coli biology.organism_classification Enterobacteriaceae Culture Media chemistry Bacteria Food Science |
| Zdroj: | Journal of Food Protection. 72:1982-1986 |
| ISSN: | 0362-028X |
| DOI: | 10.4315/0362-028x-72.9.1982 |
| Popis: | Three methods for enrichment of Shiga toxin-producing Escherichia coli (STEC) were compared using waste pit samples from swine production facilities housing 50 to 3,000 animals. The STEC gene stx2 was detected in 5 of 17 pooled samples using a U.S. Department of Agriculture (USDA) enrichment procedure, 6 of 17 samples using a U.S. Food and Drug Administration (FDA) enrichment procedure, and 8 of 17 samples using an experimental acid enrichment. All isolates were non-O157 and 5 of 6 were positive for enterotoxigenic E. coli-associated heat stable toxins a and b. The three enrichment procedures were also tested for their ability to support growth of 31 strains of STEC. The acid enrichment media supported growth of 100% of the strains, the FDA medium supported 77% of the strains, and the USDA medium supported 16% of the strains. |
| Databáze: | OpenAIRE |
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