Acute and Chronic Effects of Superior Cervical Ganglionectomy on in vitro Mitogenic Responses of Lymphocytes from Submaxillary Lymph Nodes of Pituitary-Grafted Rats
| Autor: | Ana I. Esquifino, M G García Bonacho, Patricia O. Castrillón, Agustín Arce, D. P. Cardinali |
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| Rok vydání: | 1998 |
| Předmět: |
Lipopolysaccharides
Male Pituitary gland medicine.medical_specialty Superior cervical ganglion Time Factors Surgical stress medicine.medical_treatment Superior Cervical Ganglion Lymphocyte proliferation Cellular and Molecular Neuroscience Developmental Neuroscience Anterior pituitary Internal medicine Concanavalin A Maxilla medicine Animals Lymphocytes Postoperative Period Ganglionectomy Rats Wistar business.industry Prolactin Rats medicine.anatomical_structure Endocrinology Neurology Pituitary Gland Lymph Nodes Lymph Mitogens business |
| Zdroj: | ResearcherID |
| ISSN: | 1424-8638 1424-862X |
| DOI: | 10.1159/000014525 |
| Popis: | Male rats were grafted an anterior pituitary within breast muscles or received a sham operation on day 5 of life. At the 60th day of life, the sympathetic denervation of rat submaxillary lymph nodes was achieved by a bilateral sympathetic superior cervical ganglionectomy (SCGx; at 15.00 h). Rats were killed either 18 h later (acute SCGx) or after 12 days (chronic SCGx) to measure lipopolysaccharide (LPS)- and concanavalin A (ConA)-induced cell proliferation in submaxillary lymph nodes, submaxillary lymph node cellularity and serum prolactin levels. In control rats, acute SCGx significantly augmented LPS or ConA activity on lymph cells while chronic SCGx had no effect. In pituitary-grafted rats, acute SCGx depressed the mitogenic effect of LPS or ConA whereas chronic SCGx augmented it. A global inhibitory effect of surgical stress on submaxillary lymph node cellularity was found in rats subjected to SCGx or its sham operation 18 h earlier. Serum prolactin levels increased significantly in pituitary-grafted rats, particularly in those subjected to chronic SCGx. In pituitary-grafted rats, a significant effect of acute SCGx was apparent, with serum prolactin levels augmenting about twice in sham-SCGx rats, and to a significantly less extent in acute SCGx rats. The results provide further evidence of the immunomodulatory role of local sympathetic nerves in submaxillary lymph nodes. |
| Databáze: | OpenAIRE |
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