Constitutive activation of signal transducer and activator of transcription 3 regulates expression of vascular endothelial growth factor in human meningioma differentiation
| Autor: | Zhi Gang Wang, Yun Shan Wang, Xu Zhao, Mao Xiu Zhang, Wei Ming Zhao |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Adult
Male STAT3 Transcription Factor Vascular Endothelial Growth Factor A Cancer Research Adolescent Blotting Western Biology STAT3 chemistry.chemical_compound Young Adult Constitutive activation Grade II Meningioma otorhinolaryngologic diseases Meningeal Neoplasms p-STAT3 Humans RNA Messenger neoplasms Aged Neoplasm Staging Original Paper Janus kinase 1 Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Human meningioma General Medicine Janus Kinase 1 Middle Aged Molecular biology Immunohistochemistry VEGF nervous system diseases Vascular endothelial growth factor B Vascular endothelial growth factor Vascular endothelial growth factor A Oncology chemistry Cancer research STAT protein biology.protein Disease Progression Female Meningioma Signal Transduction |
| Zdroj: | Journal of Cancer Research and Clinical Oncology |
| ISSN: | 1432-1335 |
| Popis: | Purpose Janus tyrosine kinases (JAKs) and signal transducer and activator of transcription factors (STATs), especially STAT3, are constitutively activated in human cancers. The function of STAT3 in the pathogenesis of meningioma remains unknown. In this study, we investigated the role of JAK1/STAT3 regulating vascular endothelial growth factor (VEGF) expression in the occurrence and progression of human meningioma. Methods We detected the expression of JAK1, p-JAK1, STAT3, p-STAT3, and VEGF in human meningioma and normal dura tissues by RT–PCR, Western blot analysis, and immunohistochemistry. Results JAK1, p-JAK1, STAT3, p-STAT3, and VEGF showed high expression in grade I and grade II meningioma. The level of STAT3 activation was associated with VEGF expression; all meningioma tumors that expressed p-STAT3 also expressed VEGF. Both frequency of positivity and expression were enhanced with increasing tumor grade; high frequencies and levels were found in grade II tumors, with no expression detected in normal dura tissues (P |
| Databáze: | OpenAIRE |
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