Distribution and morphology of sensory and autonomic fibres in the subendocardial plexus of the rat heart
Autor: | James F. X. Jones, Susan Pyner, Fiona C. Shenton, Thomas Campbell |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Histology Sensory Receptor Cells Tyrosine 3-Monooxygenase calcitonin gene‐related peptide Calcitonin Gene-Related Peptide synaptophysin Calcitonin gene-related peptide neurofilament Autonomic Nervous System Synaptic vesicle end‐net Choline O-Acetyltransferase 03 medical and health sciences 0302 clinical medicine Nerve Fibers tyrosine hydroxylase Animals plasma volume regulation Molecular Biology atrial mechanoreceptors Ecology Evolution Behavior and Systematics Medulla mechanotransduction biology Chemistry synaptic vesicle protein 2 Heart Cell Biology Anatomy Choline acetyltransferase Immunohistochemistry Original Papers choline acetyltransferase Atrial volume receptors Rats flower‐spray endings 030104 developmental biology Hypothalamus Synaptophysin biology.protein Cholinergic methylene blue Original Article atrial volume receptors complex unencapsulated endings 030217 neurology & neurosurgery Developmental Biology |
Zdroj: | Journal of Anatomy Journal of anatomy, 2020, Vol.238(1), pp.36-52 [Peer Reviewed Journal] |
ISSN: | 1469-7580 |
Popis: | Cardiac reflexes originating from sensory receptors in the heart ensure blood supply to vital tissues and organs in the face of constantly changing demands. Atrial volume receptors are mechanically sensitive vagal afferents which relay to the medulla and hypothalamus, affecting vasopressin release and renal sympathetic activity. To date, two anatomically distinct sensory endings have been identified which may subserve cardiac mechanosensation: end‐nets and flower‐spray endings. To map the distribution of atrial receptors in the subendocardial space, we have double‐labelled rat right atrial whole mounts for neurofilament heavy chain (NFH) and synaptic vesicle protein 2 (SV2) and generated high‐resolution maps of the rat subendocardial neural plexus at the cavo‐atrial region. In order to elucidate the nature of these fibres, double labelling with synaptophysin (SYN) and either NFH, calcitonin gene‐related peptide (CGRP), choline acetyltransferase (ChAT) or tyrosine hydroxylase (TH) was performed. The findings show that subendocardial nerve nets are denser at the superior cavo‐atrial junction than the mid‐atrial region. Adluminal plexuses had the finest diameters and stained positively for synaptic vesicles (SV2 and SYN), CGRP and TH. These plexuses may represent sympathetic post‐ganglionic fibres and/or sensory afferents. The latter are candidate substrates for type B volume receptors which are excited by stretch during atrial filling. Deeper nerve fibres appeared coarser and may be cholinergic (positive staining for ChAT). Flower‐spray endings were never observed using immunohistochemistry but were delineated clearly with the intravital stain methylene blue. We suggest that differing nerve fibre structures form the basis by which atrial deformation and hence atrial filling is reflected to the brain. In search of atrial volume receptors, we performed immunohistochemical characterisation of the subendocardial nerve plexus of the rat right atrium. Rats possess a bicuspid Eustachian valve which is richly innervated. Fine adluminal end‐nets expressed vesicular markers, CGRP and tyrosine hydroxylase. Immunohistochemistry did not label flower‐spray endings but these were identified with methylene blue. The observed plexuses could represent sympathetic post‐ganglionic and/or sensory afferents. |
Databáze: | OpenAIRE |
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