Antimicrobial potentials of Helicteres isora silver nanoparticles against extensively drug-resistant (XDR) clinical isolates of Pseudomonas aeruginosa
| Autor: | Renu Bharadwaj, Kakasaheb C. Mohite, Mansi Sharma, Varsha Shriram, Vinay Kumar, Nikunj Mapara |
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| Rok vydání: | 2015 |
| Předmět: |
Helicteres isora
Silver Respiratory chain Drug resistance Microbial Sensitivity Tests Biology medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Minimum inhibitory concentration Anti-Infective Agents Microscopy Electron Transmission Drug Resistance Multiple Bacterial Antimicrobial chemotherapy medicine Humans Pseudomonas Infections Agar diffusion test Malvaceae Pseudomonas aeruginosa Plant Extracts Spectrum Analysis General Medicine Antimicrobial biology.organism_classification Nanoparticles Biotechnology |
| Zdroj: | Applied microbiology and biotechnology. 99(24) |
| ISSN: | 1432-0614 |
| Popis: | Pseudomonas aeruginosa is a leading opportunistic pathogen and its expanding drug resistance is a growing menace to public health. Its ubiquitous nature and multiple resistance mechanisms make it a difficult target for antimicrobial chemotherapy and require a fresh approach for developing new antimicrobial agents against it. The broad-spectrum antibacterial effects of silver nanoparticles (SNPs) make them an excellent candidate for use in the medical field. However, attempts made to check their potency against extensively drug-resistant (XDR) microbes are meager. This study describes the biosynthesis and biostabilization of SNPs by Helicteres isora aqueous fruit extract and their characterization by ultraviolet-visible spectroscopy, transmission electron microscopy, dynamic light scattering, X-ray diffraction, and Fourier transform infrared spectroscopy. Majority of SNPs synthesized were of 8--20-nm size. SNPs exhibited dose-dependent antibacterial activities against four XDR P. aeruginosa (XDR-PA) clinical isolates as revealed by growth curves, with a minimum inhibitory concentration of 300 μg/ml. The SNPs exhibited antimicrobial activity against all strains, with maximum zone of inhibition (16.4 mm) in XRD-PA-2 at 1000 μg/ml. Amongst four strains, their susceptibilities to SNPs were in the following order: XDR-PA-2 > XDR-PA-4 > XDR-PA-3 > XDR-PA-1. The exposure of bacterial cells to 300 μg/ml SNPs resulted into a substantial leakage of reducing sugars and proteins, inactivation of respiratory chain dehydrogenases, and eventual cell death. SNPs also induced lipid peroxidation, a possible underlying factor to membrane porosity. The effects were more pronounced in XDR-PA-2 which may be correlated with its higher susceptibility to SNPs. These results are indicative of SNP-induced turbulence of membranous permeability as an important causal factor in XDR-PA growth inhibition and death. |
| Databáze: | OpenAIRE |
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