Transcriptional cascades during spermatogenesis: pivotal role of CREM and ACT
| Autor: | Dario De Cesare, Betina Macho, Aurore Morlon, Paolo Sassone-Corsi, Gian Maria Fimia |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Male
Transcriptional Activation endocrine system CAMP-Responsive Element Modulator Repressor Biology Biochemistry Cyclic AMP Response Element Modulator Endocrinology Coactivator Animals Humans Spermatogenesis education Molecular Biology Gene LIM domain Genetics education.field_of_study urogenital system Activator (genetics) LIM Domain Proteins Spermatozoa DNA-Binding Proteins Repressor Proteins Gene Expression Regulation Phosphorylation Homologous recombination Signal Transduction Transcription Factors |
| Zdroj: | Molecular and Cellular Endocrinology. 179:17-23 |
| ISSN: | 0303-7207 |
| DOI: | 10.1016/s0303-7207(01)00463-4 |
| Popis: | The gene CREM plays key physiological and developmental roles within the hypothalamic–pituitary–gonadal axis. We have previously shown that CREM is highly expressed in male postmeiotic cells. Spermiogenesis is a complex process by which postmeiotic male germ cells differentiate into mature spermatozoa. CREM regulates the expression of a number of post-meiotic genes involved in the process of spermiogenesis. Using homologous recombination we have generated CREM-mutant mice that display a complete block at the first step of spermiogenesis. The molecular mechanism by which CREM elicits its regulatory function involves ACT (Activator of CREM in Testis), a testis-specific coactivator constituted by a repeat of four and half LIM domains. ACT is coordinately expressed with CREM, associates with it and confers a powerful transcriptional activation function. It is able to bypass the classical requirement of CREM phosphorylation and recruiting of CBP. |
| Databáze: | OpenAIRE |
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