Specific detection of monkeypox virus by polymerase chain reaction
| Autor: | Joseph J. Esposito, Hermann Meyer, S. L. Ropp, H. Neubauer, Hans Wolf, Udo Reischl |
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| Rok vydání: | 1998 |
| Předmět: |
Male
Genes Viral viruses Cowpox Molecular Sequence Data Orthopoxvirus Poxviridae Infections Biology Polymerase Chain Reaction Inclusion Bodies Viral Frameshift mutation Open Reading Frames Viral Proteins chemistry.chemical_compound Species Specificity Virology medicine Animals Humans Poxviridae Monkeypox virus Genetics Base Sequence Cowpox virus Monkey Diseases Chromosome Mapping virus diseases Haplorhini Sequence Analysis DNA biology.organism_classification medicine.disease Open reading frame chemistry DNA Viral Vaccinia |
| Zdroj: | Journal of Virological Methods. 74:201-207 |
| ISSN: | 0166-0934 |
| DOI: | 10.1016/s0166-0934(98)00099-8 |
| Popis: | The open reading frame coding for the A-type inclusion body protein (ATI) of monkeypox virus (MPV) was identified and sequenced for two strains. Nucleotide sequence comparison revealed 72–95.3% homology with the reported open reading frame sequences of the ATIs of other orthopoxvirus species, such as variola, vaccinia, cowpox, ectromelia, and camelpox viruses. Each MPV strain contained an 8-bp deletion, which caused a frameshift that introduced a premature stop in the open reading frame at base 2091 relative to the ATI open reading frame of cowpox virus strain Brighton. The sequences enabled a primer pair to be designed that flanked the deletion and specifically amplified a 601-bp fragment that identified and differentiated 19 MPV strains examined from five other Old World orthopoxvirus species examined. The specificity was confirmed by cleavage of the 19 MPV strain amplicons with BglII, which produced three subfragments of expected sized, based on the determined MPV sequences. |
| Databáze: | OpenAIRE |
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