Removal of CD276+ cells from haploidentical memory T-cell grafts significantly lowers the risk of GVHD
| Autor: | Hans-Jörg Bühring, Karin Schilbach, Jakob Kressin, Rupert Handgretinger, Patrick Kasteleiner, Hisayoshi Hashimoto, Friederike Müller |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
T-Lymphocytes medicine.medical_treatment Graft vs Host Disease chemical and pharmacologic phenomena Hematopoietic stem cell transplantation Article Lymphocyte Depletion Mice 03 medical and health sciences 0302 clinical medicine immune system diseases In vivo medicine Animals Secretion Transplantation business.industry Haematopoietic stem cells Allotransplantation Hematopoietic Stem Cell Transplantation Hematology In vitro Peripheral surgical procedures operative 030104 developmental biology medicine.anatomical_structure Immunology business Immunologic Memory Memory T cell 030215 immunology |
| Zdroj: | Bone Marrow Transplantation |
| ISSN: | 1476-5365 0268-3369 |
| DOI: | 10.1038/s41409-021-01307-9 |
| Popis: | Detrimental graft-versus-host disease (GVHD) still remains a major cause of death in hematopoietic stem cell transplantation (HSCT). The recently explored depletion of naive cells from mobilized grafts (CD45RA depletion) has shown considerable promise, yet is unable to eliminate the incidence of GVHD. Analysis of CD45RA-depleted haploidentical mixed lymphocytes culture (haplo-MLC) revealed insufficient suppression of alloresponses in the CD4+ compartment and identified CD276 as a marker for alloreactive memory Th1 T cells. Conclusively, depleting CD276+ cells from CD45RA-depleted haplo-MLC significantly attenuated alloreactivity to recipient cells while increasing antiviral reactivity and maintaining anti-third party reactivity in vitro. To evaluate these findings in vivo, bulk, CD45RA-depleted, or CD45RA/CD276-depleted CD4+ T cells from HLA-DR4negative healthy humans were transplanted into NSG-Ab°DR4 mice, a sensitive human allo-GVHD model. Compellingly, CD45RA/CD276-depleted grafts from HLA-DR4negative donors or in vivo depletion of CD276+ cells after transplant of HLA-DR4negative memory CD4 T cells significantly delay the onset of GVHD symptoms and significantly alleviate its severity in NSG-Ab°DR4 mice. The clinical courses correlated with diminished Th1-cytokine secretion and downregulated CXCR6 expression of engrafted peripheral T cells. Collectively, mismatched HLA-mediated GVHD can be controlled by depleting recipient-specific CD276+ alloreacting T cells from the graft, highlighting its application in haplo-HSCT. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink