Characterization of the γ-secretase subunit interactome in Arabidopsis thaliana
| Autor: | Shraddha Wadurkar, Kris Gevaert, Krzysztof Leśniewicz, Geert De Jaeger, Eveline Van De Slijke, Rafal Krela, Tomasz Skrzypczak, Przemysław Wojtaszek |
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| Rok vydání: | 2019 |
| Předmět: |
0106 biological sciences
0301 basic medicine GAMMA-SECRETASE Physiology Intramembrane protease Protein subunit TANDEM AFFINITY PURIFICATION Plant Science 01 natural sciences Interactome Presenilin Protein-protein 03 medical and health sciences REGULATED INTRAMEMBRANE PROTEOLYSIS Amyloid precursor protein DISEASE RESISTANCE Integral membrane protein Gamma secretase Tandem affinity purification IDENTIFICATION biology Chemistry Presenilins Endomembrane proteins Biology and Life Sciences interactions TIR-X SELF-ASSOCIATION Cell biology AMYLOID PRECURSOR PROTEIN 030104 developmental biology CELL-DEATH PRESENILIN biology.protein Arabidopsis proteins Agronomy and Crop Science 010606 plant biology & botany |
| Zdroj: | ACTA PHYSIOLOGIAE PLANTARUM Acta Physiologiae Plantarum |
| ISSN: | 1861-1664 0137-5881 |
| DOI: | 10.1007/s11738-019-2811-3 |
| Popis: | Gamma secretase is a multi-subunit complex with aspartic intramembrane protease activity that is involved in the pathogenesis of Alzheimer’s disease in humans. In Arabidopsis thaliana, γ-secretase subunits are localized to endomembrane system compartments and interact with each other in a similar manner to their human counterparts. Here, we identified the protein partners of two plant γ-secretase subunits, presenilin 2 and PEN-2, by tandem affinity purification and co-immunoprecipitation, respectively. Integral membrane proteins were found to interact with presenilin 2, whereas secreted proteins were found to interact with PEN-2. Microscopy screening revealed that the reticulon family protein, RTNLB1, and two single transmembrane domain proteins, TIR-X and the phytolongin PHYL1.1, interact with presenilins. Finally, we show that RTNLB1 interacts with TIR-X. These results represent a step toward elucidating the functions of γ-secretase subunits in plant cells. |
| Databáze: | OpenAIRE |
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