The concentration of thiobarbituric acid reactive substances (TBARS) and paraoxonase activity in blood of patients with osteoarthrosis after endoprosthesis implantation
| Autor: | Dariusz Musiałkiewicz, Dorota Olszewska-Słonina, Kinga Lis, Bogna Kowaliszyn, Alina Woźniak, Rafał Czajkowski, Krzysztof J Olszewski, Dariusz Mątewski, Grażyna Odrowąż-Sypniewska |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: |
Adult
Male medicine.medical_specialty Erythrocytes Thiobarbituric acid Thiobarbituric Acid Reactive Substances Lipid peroxidation Prosthesis Implantation chemistry.chemical_compound Clinical Research Internal medicine Malondialdehyde Blood plasma Osteoarthritis TBARS medicine Humans endoprosthesis Aged biology business.industry Aryldialkylphosphatase Paraoxonase osteoarthrosis General Medicine Venous blood Middle Aged PON1 paraoxonase Endocrinology chemistry Case-Control Studies Immunology biology.protein Female business thiobarbituric acid |
| Zdroj: | Medical Science Monitor : International Medical Journal of Experimental and Clinical Research Dorota Olszewska-Słonina Scopus-Elsevier ResearcherID Europe PubMed Central |
| ISSN: | 1643-3750 1234-1010 |
| Popis: | Summary Background The aim of this study was to evaluate the concentration of malondialdehyde (MDA) in erythrocytes and in blood plasma and the activity of blood paraoxonase (PON1) of patients with osteoarthrosis (OA) submitted to endoprosthesis implantation for evaluating oxidative stress. Material/Methods Study was conducted on 55 patients with OA and on 54 total movement-efficient volunteers. The material for the study was venous blood plasma, serum and erythrocytes. Results Increased concentration of MDAe before surgery was observed in the group of men and in patients with a degenerative process affecting hip joints. After an implantation of endoprosthesis, MDAe decreased to the level observed in the control groups. In the study group MDA concentration in plasma was slightly lower before surgery, and after an operation it reached the value of the parameter of the reference groups. Regardless of sex or age, paraoxonase activity was almost twice as high in almost all subgroups as in the reference group. A positive correlation between PON 1 activity and MDAe concentration was demonstrated both before and after surgery in the group of men. Conclusions The increase of PON1 activity in patients’ serum in relation to the control groups indicates a probable pathogenic role of the increased formation of reactive oxygen species in the course of OA and may suggest acute inflammation of the synovial joint. The high level of PON 1 activity after endoprosthesis implantation indicates that surgical treatment may additionally stimulate ROS generation. MDAe concentration indicate more intensive process of lipid peroxidation in the elderly. |
| Databáze: | OpenAIRE |
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