Identification of Two Novel RanGTP-binding Proteins Belonging to the Importin β Superfamily
| Autor: | Maria Carmo-Fonseca, Dirk Görlich, Siegfried Prehn, Ulrike Kutay, N. Treichel, F. R. Bischoff, Regine Kraft, A. Calado, Enno Hartmann |
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| Rok vydání: | 2000 |
| Předmět: |
Molecular Sequence Data
Importin Karyopherins Biology Biochemistry DNA-binding protein 03 medical and health sciences 0302 clinical medicine Affinity chromatography medicine Animals Humans Amino Acid Sequence Nuclear pore Receptor Molecular Biology DNA Primers 030304 developmental biology 0303 health sciences Base Sequence Sequence Homology Amino Acid Nuclear Proteins Cell Biology Cell biology ran GTP-Binding Protein medicine.anatomical_structure Ran Nuclear transport Nucleus 030217 neurology & neurosurgery HeLa Cells |
| Zdroj: | The Journal of Biological Chemistry |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m006242200 |
| Popis: | Nucleo-cytoplasmic transport comprises a large number of distinct pathways, many of which are defined by members of the importin beta superfamily of nuclear transport receptors. These transport receptors all directly interact with RanGTP to modulate the compartment-specific binding of their transport substrates. To identify new members of the importin beta family, we used affinity chromatography on immobilized RanGTP and isolated Ran-binding protein (RanBP) 16 from HeLa cell extracts. RanBP16 and its close human homologue, RanBP17, are distant members of the importin beta family. Like the other members of the transport receptor superfamily, RanBP16 interacts with the nuclear pore complex and is able to enter the nucleus independent of energy and additional nuclear transport receptors. |
| Databáze: | OpenAIRE |
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