Amperometric catechol biosensor based on polyaniline–polyphenol oxidase
| Autor: | Yongyan Tan, Jinqing Kan, Jinghui Zhang, Xiaoxia Guo |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Conductometry
Inorganic chemistry Catechols Biomedical Engineering Biophysics Biosensing Techniques Sensitivity and Specificity Polyphenol oxidase chemistry.chemical_compound Aniline Polyaniline Electrochemistry Catechol oxidase Catechol biology Reproducibility of Results Equipment Design General Medicine Enzymes Immobilized Amperometry Equipment Failure Analysis chemistry Linear range biology.protein Computer-Aided Design Peptides Biosensor Catechol Oxidase Biotechnology Nuclear chemistry |
| Zdroj: | Biosensors and Bioelectronics. 25:1681-1687 |
| ISSN: | 0956-5663 |
| DOI: | 10.1016/j.bios.2009.12.007 |
| Popis: | A novel catechol biosensor was described based on the immobilization of polyphenol oxidase (PPO) into polyaniline (PANI), which was easily constructed by direct electropolymerization of aniline in a solution containing ionic liquid, 1-ethyl-3-methylimidazolium ethyl sulfate (EMIES). The developed biosensor for the detection of catechol has a linear range of 1.25–150 μmol dm−3. The maximum response current (Imax) and the Michaelis–Menten constant ( k ′ m ) are 0.62 μA and 146 μmol dm−3, respectively. The activation energy (Ea) of the PPO catalytic reaction is 31.1 kJ mol−1 in the B–R buffer. The biosensor shows good reproducibility (a relative standard deviation of 3.1% was obtained) and remarkable long-term stability (it retains 75% of the original activity after four months). The effects of potential and pH on the response current of the biosensor are also described. |
| Databáze: | OpenAIRE |
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