Draft genome sequence of Enterococcus faecium E86, a strain producing broad-spectrum antimicrobial peptides: Description of a novel bacteriocin immunity protein and a novel sequence type
| Autor: | Selda Loase Salustiano Marques-Bastos, Rodolpho Mattos Albano, Maria do Carmo de Freire Bastos, Márcia Silva Francisco, Marco Antônio Lemos Miguel, Felipe Miceli de Farias, Ilana Nascimento de Sousa Santos |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Microbiology (medical) Enterococcus faecium 030106 microbiology Immunology Sequence assembly Microbial Sensitivity Tests Microbiology Genome Vancomycin-Resistant Enterococci 03 medical and health sciences 0302 clinical medicine Bacteriocins Bacteriocin Drug Resistance Bacterial Humans Immunology and Allergy 030212 general & internal medicine Gene Bacteriocin immunity Genetics Whole genome sequencing Whole Genome Sequencing biology food and beverages Sequence Analysis DNA Genome project biology.organism_classification Listeria monocytogenes Anti-Bacterial Agents Multigene Family Genome Bacterial Antimicrobial Cationic Peptides |
| Zdroj: | Journal of Global Antimicrobial Resistance. 17:195-197 |
| ISSN: | 2213-7165 |
| DOI: | 10.1016/j.jgar.2019.04.004 |
| Popis: | Objectives The aim of this study was to report the draft genome sequence of the bacteriocinogenic strain Enterococcus faecium E86. Bacteriocins are prokaryotic peptides or proteins with antimicrobial activity. The genome information may contribute to the identification of enterocins produced by this strain that exhibit inhibitory activity against the foodborne pathogen Listeria monocytogenes and vancomycin-resistant enterococci (VRE) involved in human infections, among other bacterial genera and species. Methods An Illumina MiSeq platform was used for genome sequencing. De novo assembly of 5 735 838 paired-end reads was done using the A5-miseq pipeline, yielding >300-fold average genome coverage. Genome annotation was performed by the RAST server, and mining of the bacteriocinogenic gene clusters was done using the BAGEL3 and antiSMASH v.4 platforms. Results The total scaffold size was determined to be 2 689 107 bp, approximately 2.7 Mbp, featuring a G + C content of 38.1%. The genome contains 2858 coding sequences and 74 RNA genes. Genome analyses revealed the presence of: 30 genes involved in drug resistance; 2 bacteriocinogenic gene clusters (for enterocin P and enterocin TW21); EntiTW21, a novel bacteriocin immunity protein and a novel multilocus sequence type (ST1500). Conclusion This work highlights the potential biotechnological application of this strain for the production of enterocin P, a bacteriocin that can be employed in the food industry as a biopreservative against L. monocytogenes and as an alternative to classical antibiotics against VRE. |
| Databáze: | OpenAIRE |
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