Biofilm Bacteria Use Stress Responses to Detect and Respond to Competitors
| Autor: | Stefanie Roberfroid, David De Coster, Lise Dieltjens, Hans Steenackers, Kevin R. Foster, Bram Lories |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Salmonella typhimurium
0301 basic medicine Multidrug tolerance media_common.quotation_subject Virulence General Biochemistry Genetics and Molecular Biology Competition (biology) 03 medical and health sciences 0302 clinical medicine Drug Resistance Bacterial Type VI secretion system media_common biology Biofilm Biofilm matrix biology.organism_classification Cell biology Phenotype 030104 developmental biology Biofilms Microbial Interactions Efflux Single-Cell Analysis General Agricultural and Biological Sciences Genome Bacterial 030217 neurology & neurosurgery Bacteria |
| Zdroj: | Current Biology |
| ISSN: | 0960-9822 |
| DOI: | 10.1016/j.cub.2020.01.065 |
| Popis: | Bacteria use complex regulatory networks to cope with stress, but the function of these networks in natural habitats is poorly understood. The competition sensing hypothesis states that bacterial stress response systems can serve to detect ecological competition, but studying regulatory responses in diverse communities is challenging. Here, we solve this problem by using differential fluorescence induction to screen the Salmonella Typhimurium genome for loci that respond, at the single-cell level, to life in biofilms with competing strains of S. Typhimurium and Escherichia coli. This screening reveals the presence of competing strains drives up the expression of genes associated with biofilm matrix production (CsgD pathway), epithelial invasion (SPI1 invasion system), and, finally, chemical efflux and antibiotic tolerance (TolC efflux pump and AadA aminoglycoside 3-adenyltransferase). We validate that these regulatory changes result in the predicted phenotypic changes in biofilm, mammalian cell invasion, and antibiotic tolerance. We further show that these responses arise via activation of major stress responses, providing direct support for the competition sensing hypothesis. Moreover, inactivation of the type VI secretion system (T6SS) of a competitor annuls the responses to competition, indicating that T6SS-derived cell damage activates these stress response systems. Our work shows that bacteria use stress responses to detect and respond to competition in a manner important for major phenotypes, including biofilm formation, virulence, and antibiotic tolerance. ispartof: Current Biology vol:30 issue:7 pages:1231-1244 ispartof: location:England status: published |
| Databáze: | OpenAIRE |
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