MiR-205 mediated APC regulation contributes to pancreatic cancer cell proliferation
| Autor: | Rui-Feng Qin, Hao-Ran Huo, Jia-Dong Xue, Jia Zhang, Zeng-Jiang Yuan |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
MiR-205
Male Microarray Adenomatous polyposis coli Proliferation Adenomatous Polyposis Coli Protein Primary Cell Culture Down-Regulation 03 medical and health sciences 0302 clinical medicine Western blot Pancreatic cancer microRNA Biomarkers Tumor Tumor Cells Cultured medicine Humans Pancreas Aged Cell Proliferation Oligonucleotide Array Sequence Analysis Aged 80 and over biology medicine.diagnostic_test Microarray analysis techniques Cell growth Gene Expression Profiling Gastroenterology General Medicine Basic Study Middle Aged Prognosis medicine.disease Up-Regulation Gene Expression Regulation Neoplastic Pancreatic Neoplasms MicroRNAs 030220 oncology & carcinogenesis biology.protein Cancer research Female 030211 gastroenterology & hepatology Signal transduction |
| Zdroj: | World Journal of Gastroenterology |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.v25.i28.3775 |
| Popis: | Background Pancreatic cancer is a deadly malignancy with aggressive properties. MicroRNAs (miRNAs) participate in the pathogenesis of a variety of diseases and molecular processes by targeting functional mRNAs. Nevertheless, the regulatory role of miRNAs in signaling pathways involved in pancreatic cancer remains largely unknown. Aim To explore the molecular regulation involved in pancreatic cancer and potential mechanisms of miR-205. Methods Microarray analysis was performed to investigate the expression profile of miRNAs in pancreatic cancer. Expression of miR-205 was validated by qRT-PCR. Target prediction and functional enrichment analysis were employed to seek potential target genes of miR-205 and potential functions of these genes. The target binding of miR-205 and adenomatous polyposis coli (APC) was validated by luciferase reporter assay. APC protein expression in pancreatic cancer was validated by qRT-PCR and Western blot. Proliferation was evaluated by MTT and colony formation assays. Results A large number of miRNAs with altered expression were identified in pancreatic cancer. MiR-205 was significantly up-regulated. APC was found to be a validated target of miR-205 and down-regulated in pancreatic cancer. Proliferation experiments showed that miR-205 could promote cell proliferation in pancreatic cancer by targeting APC. Conclusion The above findings suggested that miR-205 mediated APC regulation contributes to pancreatic cancer development, which could be considered as a novel prognostic biomarker for clinical care. |
| Databáze: | OpenAIRE |
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