Exposure to a firefighting overhaul environment without respiratory protection increases immune dysregulation and lung disease risk
| Autor: | Vincent L. Tir, Kenneth W. Fent, Denise L. Smith, Gregory G. Freund, Jenny Drnevich, Stephen J. Gainey, Gavin P. Horn, M.L. Oelschlager, Albert E. Towers, Stephen Kerber |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Lung Diseases Male Pulmonology Physiology Molecular biology Gene Expression lcsh:Medicine medicine.disease_cause Biochemistry Cohort Studies 0302 clinical medicine Sequencing techniques RAR-related orphan receptor gamma Risk Factors Immune Physiology Medicine and Health Sciences Respiratory system Respiratory Protective Devices lcsh:Science Lung Inhalation Exposure Multidisciplinary Immune System Proteins Mammalian Genomics RNA sequencing Animal Models Genomics 030210 environmental & occupational health medicine.anatomical_structure Experimental Organism Systems Immune System Diseases Models Animal Engineering and Technology Research Article Immunology Mouse Models Air Pollutants Occupational Environmental and Occupational Lung Diseases Research and Analysis Methods Antibodies Fires 03 medical and health sciences Immune system Model Organisms Downregulation and upregulation Occupational Exposure DNA-binding proteins medicine Genetics Animals Carcinogen Smoke business.industry Fire Suppression Technology lcsh:R Biology and Life Sciences Proteins Immune dysregulation Mice Inbred C57BL Fire Engineering 030104 developmental biology Molecular biology techniques Gene Expression Regulation Animal Genomics Firefighters lcsh:Q business Transcriptome |
| Zdroj: | PLoS ONE, Vol 13, Iss 8, p e0201830 (2018) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Firefighting activities appear to increase the risk of acute and chronic lung disease, including malignancy. While self-contained breathing apparatuses (SCBA) mitigate exposures to inhalable asphyxiates and carcinogens, firefighters frequently remove SCBA during overhaul when the firegrounds appear clear of visible smoke. Using a mouse model of overhaul without airway protection, the impact of fireground environment exposure on lung gene expression was assessed to identify transcripts potentially critical to firefighter-related chronic pulmonary illnesses. Lung tissue was collected 2 hrs post-overhaul and evaluated via whole genome transcriptomics by RNA-seq. Although gas metering showed that the fireground overhaul levels of carbon monoxide (CO), carbon dioxide (CO2), hydrogen cyanine (HCN), hydrogen sulfide (H2S) and oxygen (O2) were within NIOSH ceiling recommendations, 3852 lung genes were differentially expressed when mice exposed to overhaul were compared to mice on the fireground but outside the overhaul environment. Importantly, overhaul exposure was associated with an up/down-regulation of 86 genes with a fold change of 1.5 or greater (p |
| Databáze: | OpenAIRE |
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