Differences in expression of retinal proteins between diabetic and normal rats
| Autor: | Cheng-Jun Li, Jian Kang, Rong Cai, En-Jie Tang, Hui-Jun Yang, Shang-Qing Liu, Bin Wen |
|---|---|
| Rok vydání: | 2007 |
| Předmět: |
Guanylate kinase
Protein subunit Diabetes Mellitus Experimental Rats Sprague-Dawley Random Allocation chemistry.chemical_compound Tandem Mass Spectrometry Crystallin Alloxan Animals Eye Proteins Diabetic Retinopathy ATP synthase biology Gene Expression Profiling Gastroenterology Retinal General Medicine Rats Disease Models Animal Biochemistry chemistry biology.protein Electrophoresis Polyacrylamide Gel Female Isoelectric Focusing Retinal Rod Photoreceptor Cells Signal transduction Rapid Communication |
| Zdroj: | World Journal of Gastroenterology. 13:2118 |
| ISSN: | 1007-9327 |
| DOI: | 10.3748/wjg.v13.i14.2118 |
| Popis: | AIM: To compare and identify the differences in expression of retinal proteins between normal and diabetic rats, and to analyze the molecular pathogenetic mechanisms of retinal diseases caused by diabetes. METHODS: Changes in protein expression of retinal tissues from diabetic and normal rats were observed using 2-dimensional polyacrylamide gel electrophoresis (2-DE). Some protein spots exhibiting statistically significant variations (P < 0.05) were selected randomly and identified by tandem mass spectrometry and analyzed by bioinformatics. RESULTS: 2-DE showed that the expression was up-regulated in 5 retinal proteins, down-regulated in 23 retinal proteins, and disappeared in 8 retinal proteins. Eight spots were identified from the 36 spots by tandem mass spectrometry (MS/MS) and analyzed by bioinformatics. Guanylate kinase 1, triosephosphate isomerase 1, ATP synthase subunit d, albumin and dimethylarginine dimethylaminohydrolase 2 played an important role in signal transduction. Triosephosphate isomerase 1, crystallin alpha B, ATP synthase subunit d and peroxiredoxin 6 were involved in energy metabolism of retinal tissues. Guanylate kinase 1 played an important role in photoexcitation of retinal rod photoreceptor cells. Whether crystallin beta A1 plays a role in diabetic retinas is unknown so far. CONCLUSION: There are differences in expression of retinal proteins between diabetic and normal rats. These proteins may be involved in the mechanisms and prognosis of retinal diseases caused by diabetes. |
| Databáze: | OpenAIRE |
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