The origin and genetic diversity of the causal agent of Asian soybean rust,Phakopsora pachyrhizi, in South America
| Autor: | Eduardo A. Guillin, Márcio Ribeiro Silva, Luiz Orlando de Oliveira, V.R. Jorge, Ivan Schuster, Maíra Cristina Menezes Freire, Álvaro M. R. Almeida |
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| Rok vydání: | 2014 |
| Předmět: |
Molecular Genetic Variation
Soja América del Sur Plant Science Horticulture Roya de la Soja Variación Genética Roya Genetic variation Genotype Botany Enfermedades de las Plantas Genetics Internal transcribed spacer Plant Diseases Genetic diversity Phakopsora pachyrhizi biology Diversidad Genética Genetic Variation food and beverages South America biology.organism_classification Rusts Housekeeping gene Genetic structure Roya Asiática Soybeans Agronomy and Crop Science |
| Zdroj: | Plant Pathology 64 (3) : 729-737 (June 2015) INTA Digital (INTA) Instituto Nacional de Tecnología Agropecuaria instacron:INTA |
| ISSN: | 0032-0862 |
| DOI: | 10.1111/ppa.12300 |
| Popis: | A sequence‐based approach was used to investigate molecular genetic variations in Phakopsora pachyrhizi, an obligate biotrophic pathogen that causes Asian soybean rust. In Argentina, the samples came from uredinium‐bearing leaves taken from 11 soybean fields; in Brazil, the samples comprised urediniospores from leaves of 10 soybean genotypes that had been grown in three experimental stations during two growing seasons. PCR‐based cloning techniques were used to generate DNA sequences for two gene regions and alignments were supplemented with data from GenBank. A total of 575 sequences for the internal transcribed spacer region (18 ribotypes) and 160 partial sequences for a housekeeping gene encoding ADP‐ribosylation factor (10 haplotypes) were obtained. Ribotype accumulation curves predicted that about 20 bacterial clones would recover 5–6 ribotypes (c. 70–80% of the total molecular variation) per locality. The samples from the three experimental stations in Brazil displayed most (14 out of 16) ribotypes found worldwide; the lack of genetic structure and differentiation at a diverse geographic scale suggests that both local and distant sources provide airborne inoculum during disease establishment. Soybean genotypes with resistance genes for the Asian soybean rust did not decrease the molecular genetic variation of fungal populations. Instituto de Genética Fil: Jorge, V.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil Fil: Silva, M.R. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil Fil: Guillin, Eduardo Alejandro. Instituto Nacional de Tecnología Agropecuaria (INTA). Instituto de Genética; Argentina Fil: Freire, M.C.M. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil Fil: Schuster, I. Cooperativa Central de Pesquisa Agrícola; Brasil Fil: Almeida, A.M.R. Empresa Brasileira de Pesquisa Agropecuária, Embrapa‐Soja; Brasil Fil: Oliveira, Luiz O. Universidade Federal de Viçosa. Departamento de Bioquímica e Biologia Molecular; Brasil |
| Databáze: | OpenAIRE |
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