Antiulcer and Antioxidant Activity of a Lectin from Mucuna pruriens Seeds on Ethanol- induced Gastropathy: Involvement of Alpha-2 Adrenoceptors and Prostaglandins
| Autor: | Karuza Maria Alves Pereira, Isabela Ribeiro Pinto, Hellíada Vasconcelos Chaves, Carlos Alberto de Almeida Gadelha, Gerardo Cristino Filho, Mirna Marques Bezerra, Francisca Cléa Florenço de Sousa, Vicente de Paulo Teixeira Pinto, Kátia Alves Ribeiro, Ana Flávia Seraine Custódio Viana, Lara Mendes Maciel, José Thalles Jocelino Gomes de Lacerda, Tatiane Santi-Gadelha, Samuel Mateus Pereira Filho, Auriana Serra Vasconcelos, Raul Sousa Freitas, Antonio Alfredo Rodrigues e Silva |
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| Rok vydání: | 2019 |
| Předmět: |
Antioxidant
medicine.medical_treatment Pharmacology Antioxidants Lipid peroxidation Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Lectins Edema Drug Discovery Toxicity Tests Acute Animals Medicine Stomach Ulcer 030304 developmental biology 0303 health sciences Ethanol biology Plant Extracts business.industry Glutathione biology.organism_classification Mucuna Acute toxicity Receptors Adrenergic Yohimbine chemistry Gastric Mucosa 030220 oncology & carcinogenesis Seeds Toxicity Prostaglandins Lipid Peroxidation medicine.symptom business Mucuna pruriens Phytotherapy medicine.drug |
| Zdroj: | Current Pharmaceutical Design. 25:1430-1439 |
| ISSN: | 1381-6128 |
| DOI: | 10.2174/1381612825666190524081433 |
| Popis: | Ethnopharmacological Relevance:Mucuna pruriens (Mp) belongs to Leguminosae family, it is native of tropical regions and used to treat several maladies such as urinary, neurological, and menstruation disorders, constipation, edema, fever, tuberculosis, ulcers, diabetes, arthritis, dysentery, and cardiovascular diseases. Mp seeds are rich in bioactive compounds, for instance, lectins, a heterogeneous group of proteins and glycoproteins with a potential role as therapeutic tools for several conditions, including gastric disorders. This study investigated the acute toxicity, gastroprotective, and antioxidant activities of a lectin from Mucuna pruriens seeds (MpLec) on ethanol-induced gastropathy model in mice.Materials & Methods:Mice received MpLec (5 or 10 mg/kg; i.v.) and were observed for acute toxicity signs; in another experimental series, mice were pre-treated with MpLec (0.001; 0.01 or 0.1 mg/kg, i.v.), ranitidine (80 mg/kg, p.o.), or saline (0.3 mL/30g, i.v.) before ethanol 99.9% (0.2 mL/animal, p.o.), and euthanized 30 min after ethanol challenge. Macroscopic and microscopic gastric aspects, biochemical parameters (tissue hemoglobin levels, iron-induced lipid peroxidation, GSH content, SOD activity, and gastric mucosal PGE2) were measured. Additionally, pharmacological tools (yohimbine, indomethacin, naloxone, L-NAME) were opportunely used to clarify MpLec gastroprotective mechanisms of action.Results:No toxicity signs nor death were observed at acute toxicity tests. MpLec reduced ethanol-induced gastric damage, edema, and hemorrhagic patches formation, as well as decreased lipid peroxidation, SOD activity, and increased GSH content. Yohimbine and indomethacin prevented MpLec effects, suggesting the involvement of alpha-2 adrenoceptors and prostaglandins in the MpLec-mediated effects.Conclusion:MpLec does not present toxicity signs and shows gastroprotective and antioxidant activities via alpha-2 adrenoceptors and prostaglandins in the ethanol-induced gastropathy model. |
| Databáze: | OpenAIRE |
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