Assay of the synthetic estrogen fosfestrol in pharmaceutical formulations using capillary electrophoresis
| Autor: | Anastasios V. Trellopoulos, Demetrius G. Themelis, Paraskevas D. Tzanavaras, Bo Karlberg |
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| Rok vydání: | 2005 |
| Předmět: |
Male
Analyte Fosfestrol Time Factors Antineoplastic Agents Hormonal Drug Industry Clinical Biochemistry Analytical chemistry Pharmaceutical Science Electrolyte Pharmaceutical formulation Dosage form Analytical Chemistry Capillary electrophoresis Drug Discovery medicine Humans Diethylstilbestrol Chromatography High Pressure Liquid Spectroscopy Detection limit Chromatography Chemistry Electrophoresis Capillary Prostatic Neoplasms Reproducibility of Results Estrogens Electrophoresis Models Chemical Pharmaceutical Preparations medicine.drug |
| Zdroj: | Journal of Pharmaceutical and Biomedical Analysis. 39:559-563 |
| ISSN: | 0731-7085 |
| Popis: | This study reports – for the first time – a capillary electrophoretic method for the determination of fosfestrol, a synthetic estrogen used in the treatment of metastatic prostate cancer. The effects of the carrier ion concentration, injected volume and applied voltage were studied and optimized. A 10 mM sodium tetraborate solution was selected as the carrier electrolyte solution, while the sample was injected hydrodynamically by applying a 20 mmHg vacuum for 1 s. The driving voltage was 30 kV and the absorbance of the analyte (peak height) was monitored at 240 nm. Under the above-mentioned conditions, the migration time of fosfestrol was 6.6 min. Linearity was achieved in the analyte range 3–150 mg L −1 with the detection limit being 1 mg L −1 . The proposed method is adequately precise ( s r = 2.8% at 100 mg L −1 fosfestrol, n = 10) without the use of an internal standard and was applied to the determination of fosfestrol in a pharmaceutical formulation. The results obtained by the proposed method were in good agreement with those derived from the USP reference method. |
| Databáze: | OpenAIRE |
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