Alpha-Amylase and Alpha-Glucosidase Enzyme Inhibition and Antioxidant Potential of 3-Oxolupenal and Katononic Acid Isolated from Nuxia oppositifolia
| Autor: | Mohamed F. Alajmi, Shaza M. Al-Massarani, Rabab A. El Dib, Ali A. El-Gamal, Valentina Razmovski-Naumovski, Tabish Rehman, Mohammed S. Alqahtani, Syed Hidayathulla, Ali S Alqahtani |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
glucosidase
amylase antioxidant 3-oxolupenal Antioxidant DPPH medicine.medical_treatment lcsh:QR1-502 nuxia oppositifolia dpph Biochemistry lcsh:Microbiology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Column chromatography abts medicine Amylase Molecular Biology 030304 developmental biology chemistry.chemical_classification 0303 health sciences Chromatography ABTS biology katononic acid Enzyme chemistry Alpha-glucosidase 030220 oncology & carcinogenesis docking biology.protein Alpha-amylase |
| Zdroj: | Biomolecules, Vol 10, Iss 1, p 61 (2019) Biomolecules Volume 10 Issue 1 |
| ISSN: | 2218-273X |
| DOI: | 10.3390/biom10010061 |
| Popis: | Nuxia oppositifolia is traditionally used in diabetes treatment in many Arabian countries however, scientific evidence is lacking. Hence, the present study explored the antidiabetic and antioxidant activities of the plant extracts and their purified compounds. The methanolic crude extract of N. oppositifolia was partitioned using a two-solvent system. The n-hexane fraction was purified by silica gel column chromatography to yield several compounds including katononic acid and 3-oxolupenal. Antidiabetic activities were assessed by &alpha amylase and &alpha glucosidase enzyme inhibition. Antioxidant capacities were examined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2&prime azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) scavenging assays. Further, the interaction between enzymes (&alpha glucosidase) and ligands (3-oxolupenal and katononic acid) was followed by fluorescence quenching and molecular docking studies. 3-oxolupenal and katononic acid showed IC50 values of 46.2 &mu g/mL (101.6 µ M) and 52.4 &mu g/mL (119.3 µ M), respectively against the amylase inhibition. 3-oxolupenal (62.3 µ g/mL or 141.9 &mu M) exhibited more potent inhibition against &alpha glucosidases compared to katononic acid (88.6 µ g/mL or 194.8 &mu M). In terms of antioxidant activity, the relatively polar crude extract and n-butanol fraction showed the greatest DPPH and ABTS scavenging activity. However, the antioxidant activities of the purified compounds were in the low to moderate range. Molecular docking studies confirmed that 3-oxolupenal and katononic acid interacted strongly with the active site residues of both &alpha glucosidase. Fluorescence quenching results also suggest that 3-oxolupenal and katononic acid have a good affinity towards both &alpha glucosidase enzymes. This study provides preliminary data for the plant&rsquo s use in the treatment of type 2 diabetes mellitus. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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