Molecular cloning of DAX1 and SHP cDNAs and their expression patterns in the Nile tilapia, Oreochromis niloticus

Autor: Cheni Chery Sudhakumari, Ken Ichirou Morohashi, Masaru Matsuda, Yoshitaka Nagahama, De Shou Wang, Tohru Kobayashi, Michiyasu Yoshikuni, Fumie Sakai, Taiga Suzuki, Balasubramanian Senthilkumaran
Rok vydání: 2002
Předmět:
animal structures
Receptors
Retinoic Acid
Molecular Sequence Data
Biophysics
Receptors
Cytoplasmic and Nuclear
chemical and pharmacologic phenomena
Molecular cloning
Biochemistry
Nile tilapia
Animals
Humans
Amino Acid Sequence
Northern blot
Cloning
Molecular
Molecular Biology
Gene
Phylogeny
DNA Primers
Expressed Sequence Tags
Mammals
Regulation of gene expression
Expressed sequence tag
Base Sequence
Sequence Homology
Amino Acid
biology
DAX-1 Orphan Nuclear Receptor
hemic and immune systems
Cell Biology
biology.organism_classification
Molecular biology
Actins
Introns
Recombinant Proteins
DNA-Binding Proteins
Repressor Proteins
Open reading frame
Oreochromis
Gene Expression Regulation
embryonic structures
biological phenomena
cell phenomena
and immunity
Sequence Alignment
Tilapia
Transcription Factors
Zdroj: Biochemical and Biophysical Research Communications. 297:632-640
ISSN: 0006-291X
DOI: 10.1016/s0006-291x(02)02252-0
Popis: Piscine DAX1 and SHP cDNAs with an open reading frame encoding 296 and 258 amino acid residues, respectively, as well as SHP partial gene fragment, were cloned from Nile tilapia. Phylogenetic analyses of DAX1s, SHPs, and homologous EST fragments indicate that DAX1 and SHP are conserved in gene structure and are present throughout vertebrates. A single band of approximately 1.4kb for DAX1 and of approximately 1.2kb for SHP was detected in the Northern blot analysis. Tissue distribution analysis by RT-PCR showed that fish DAX1 and SHP mRNAs are widely expressed in adult tissues, with the most abundant expression in gonads and liver, respectively. DAX1 and SHP were also detected in gonads of both sexes at 5-90 days after hatching (dah). However, the expression of DAX1 is weak at 5 and 10dah and then significantly up-regulated between 10 and 15dah, whereas the expression of SHP is moderate and consistent during the ontogeny.
Databáze: OpenAIRE
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