T-cell regulation of immunoglobulin synthesis and proliferation in pokeweed (Pa-1)-stimulated human lymphocyte cultures
| Autor: | T A Platts-Mills, M. J. Snajdr, G J Janossy, E. Gomez de la Concha, A. Luquetti, M. J. Waxdal |
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| Rok vydání: | 1977 |
| Předmět: |
Cell Survival
T cell T-Lymphocytes Immunology Cell Population Immunoglobulins Stimulation Cell Separation Lymphocyte Activation Monocytes Leukocyte Count medicine Cytotoxic T cell Humans Secretion education Cells Cultured education.field_of_study B-Lymphocytes biology Cell Membrane General Medicine Fibroblasts Molecular biology Culture Media Immunoglobulin A medicine.anatomical_structure Immunoglobulin M Tonsil Immunoglobulin G biology.protein Antibody Mitogens Cell Division |
| Zdroj: | Scandinavian journal of immunology. 6(1-2) |
| ISSN: | 0300-9475 |
| Popis: | In pokeweed (Pa-1)-stimulated human lymphocyte cultures, T cells are essential for the survival, proliferation, plasma-cell development, and high-rate Ig secretion of B cells. Their effects are T-cell-specific in the sense that B-cell stimulation does not take place in the absence of T cells even when fibroblasts or monocytes are provided. The experimental system is the most effective model for activation of human B lymphocytes so far described. Plasmablast development requires approximately 7 days in culture. In T + B-cell cultures established at 1 X 10(6)/ml (1 X 10(4)/mm2) initial cell density, plasma cells can secrete, on the average, as much as 40-70 pg IgM or IgG per cell per day. When the initial T-cell density is increased well above 1.0 X 10(6)/ml, a T-cell-mediated depression of Ig synthesis becomes predominant. Thus, in the pokeweed model T-cell effects represent a balance of helper and suppressor influences. The study also shows that B cells are heterogeneous. A non-adherent IgG-committed (smIg-?) TONSIL B-cell population seems to be less susceptible to T suppressor effects than normal tonsil B cells. This subset proliferates particularly well and synthesizes large quantities of IgG in the presence of large initial proportions of T cells. |
| Databáze: | OpenAIRE |
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