Human papillomavirus detection and p16INK4a expression in cervical lesions: a comparative study
| Autor: | Gemma Mancebo, Ramon Carreras, Mercè Muset, Maria Teresa Baró, Jordi Genovés, Belen Lloveras, Beatriz Casado, Silvia Agramunt, Laia Serrano, Frances Alameda, Esther Miralpeix, Josep Maria Sole, Beatriz Bellosillo |
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| Rok vydání: | 2014 |
| Předmět: |
Adult
Pathology medicine.medical_specialty viruses Uterine Cervical Neoplasms urologic and male genital diseases Cervical intraepithelial neoplasia Pathology and Forensic Medicine Lesion Young Adult Biomarkers Tumor medicine Humans Papillomaviridae Young adult neoplasms Genotyping Cyclin-Dependent Kinase Inhibitor p16 biology Reverse Transcriptase Polymerase Chain Reaction business.industry Papillomavirus Infections virus diseases Middle Aged Uterine Cervical Dysplasia medicine.disease biology.organism_classification Immunohistochemistry female genital diseases and pregnancy complications Koilocyte Dysplasia Female medicine.symptom business |
| Zdroj: | Human Pathology. 45:826-833 |
| ISSN: | 0046-8177 |
| DOI: | 10.1016/j.humpath.2013.10.035 |
| Popis: | p16(INK4a) expression in dysplastic cervical lesions is related to high-risk human papillomavirus (HR-HPV) infection. The immunohistochemical expression of this protein in these lesions allows an increase in diagnostic reproducibility in biopsies and the introduction of prognostic factors in low-grade lesions. Here, we studied the immunohistochemical expression of p16 in 86 dysplastic cervical lesions, 54 cervical intraepithelial neoplasms-grade 1 (CIN-I), 23 CIN-II, and 9 CIN-III. In addition, we performed HPV detection and genotyping. We detected HR-HPV in 19/54 CIN-I, 21/23 CIN-II and 9/9 CIN-III cases. p16(INK4a) immunoreactivity was observed in 7/19 CIN-I HR-HPV-positive, 17/21 CIN-II HR-HPV-positive and all CIN-III cases. Immunoreactivity for p16(INK4a) was found in 7/54 CIN-I and in 17/23 CIN-II cases. In the follow-up, we detected 3 p16-positive high-grade squamous epithelial lesions (CIN-II and CIN-III) in the CIN-I/p16-negative group and 5 p16-positive high-grade squamous epithelial lesions cases in the CIN-II/p16-negative group. We conclude that p16 negativity in CIN-I and CIN-II biopsies does not always imply regression of the lesion and that the diagnosis of CIN-II should not be based solely on p16 results. |
| Databáze: | OpenAIRE |
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