Characterization and bioactivities of a novel polysaccharide obtained from Gracilariopsis lemaneiformis

Autor: Gui-Qing Sun, Chen-Shan Shi, Ya-Xin Sang, Xiang-Hong Wang, Tian-Ye Li, Zheng-Si Gong
Rok vydání: 2016
Předmět:
food.ingredient
Magnetic Resonance Spectroscopy
bioactivities
Chemical structure
Enzyme-Linked Immunosorbent Assay
02 engineering and technology
Biology
Polysaccharide
01 natural sciences
chemistry.chemical_compound
Mice
Column chromatography
food
Polysaccharides
Reference Values
Spectroscopy
Fourier Transform Infrared
Splenocyte
Agar
Animals
Lymphocytes
lcsh:Science
Sugar
Chromatography
High Pressure Liquid
Cell Proliferation
chemistry.chemical_classification
Multidisciplinary
Molecular Structure
010405 organic chemistry
Periodic Acid
structural characterization
Glycosidic bond
021001 nanoscience & nanotechnology
Seaweed
0104 chemical sciences
Gracilariopsis lemaneiformis
Molecular Weight
chemistry
Biochemistry
DEAE-Sepharose
polysaccharide
Rhodophyta
Microscopy
Electron
Scanning
lcsh:Q
0210 nano-technology
Zdroj: Anais da Academia Brasileira de Ciências v.89 n.1 2017
Anais da Academia Brasileira de Ciências
Academia Brasileira de Ciências (ABC)
instacron:ABC
Anais da Academia Brasileira de Ciências, Volume: 89, Issue: 1, Pages: 175-189, Published: MAR 2017
Anais da Academia Brasileira de Ciências, Vol 89, Iss 1, Pp 175-189
ISSN: 1678-2690
Popis: Gracilariopsis lemaneiformis is a type of red alga that contains seaweed polysaccharide agar. In this study, a novel non-agar seaweed polysaccharide fraction named GCP (short of crude polysaccharide obtained from Gracilariopsis lemaneiformis) was isolated from Gracilariopsis lemaneiformis. Structural analysis showed that GCP shows triple helical chain conformation when dissolved in water and has many branches and long side chains. Also, 1→3 linkage is the major linkage and the sugar structures are galactopyranose configurations linked by β-type glycosidic linkages. Two macromolecular substance fractions (GCP-1 and GCP-2) were purified by DEAE Sepharose Fast Flow column chromatography. Moreover, a splenocyte damage assay and splenocyte proliferation assay were used to analyse the bioactivities of GCP, GCP-1 and GCP-2. It was demonstrated that polysaccharides could protect splenocyte damaged by H2O2; GCP-2 shows a greatest protection rate, that is, 92.8%, which significantly enhanced the splenocyte proliferation, and GCP showed the highest proliferation rate, 9.30%. The results suggested that this type of novel non-agar polysaccharide displayed remarkable antioxidant and immunomodulatory activities and early alkali treatment could decrease the activities. It may represent a potential material for health food and clinical medicines.
Databáze: OpenAIRE
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