Microscopic multifrequency MR elastography for mapping viscoelasticity in zebrafish
| Autor: | Tom Meyer, Heiko Tzschätzsch, Jakob Ernst Luis Jordan, Ingolf Sack, Luca Bramè, Anton Gauert, Jürgen Braun, Yasmine Safraou, Leif Schröder, Helge Herthum, Anja I. H. Hagemann, Gergely Bertalan |
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| Rok vydání: | 2021 |
| Předmět: |
tumors
Viscoelasticity neuroblastoma stiffness Animal model Nuclear magnetic resonance Reference Values medicine Animals Radiology Nuclear Medicine and imaging Zebrafish viscoelasticity MR elastography Physics biology medicine.diagnostic_test Viscosity Brain Wave speed Optic tectum zebrafish biology.organism_classification Reference values Elasticity Imaging Techniques Elastography 600 Technik Medizin angewandte Wissenschaften::610 Medizin und Gesundheit::610 Medizin und Gesundheit |
| Zdroj: | Magnetic Resonance in Medicine. 87:1435-1445 |
| ISSN: | 1522-2594 0740-3194 |
| Popis: | Purpose: The zebrafish (Danio rerio) has become an important animal model in a wide range of biomedical research disciplines. Growing awareness of the role of biomechanical properties in tumor progression and neuronal development has led to an increasing interest in the noninvasive mapping of the viscoelastic properties of zebrafish by elastography methods applicable to bulky and nontranslucent tissues. Methods: Microscopic multifrequency MR elastography is introduced for mapping shear wave speed (SWS) and loss angle (φ) as markers of stiffness and viscosity of muscle, brain, and neuroblastoma tumors in postmortem zebrafish with 60 µm in-plane resolution. Experiments were performed in a 7 Tesla MR scanner at 1, 1.2, and 1.4 kHz driving frequencies. Results: Detailed zebrafish viscoelasticity maps revealed that the midbrain region (SWS = 3.1 ± 0.7 m/s, φ = 1.2 ± 0.3 radian [rad]) was stiffer and less viscous than telencephalon (SWS = 2.6 ± 0. 5 m/s, φ = 1.4 ± 0.2 rad) and optic tectum (SWS = 2.6 ± 0.5 m/s, φ = 1.3 ± 0.4 rad), whereas the cerebellum (SWS = 2.9 ± 0.6 m/s, φ = 0.9 ± 0.4 rad) was stiffer but less viscous than both (all p < .05). Overall, brain tissue (SWS = 2.9 ± 0.4 m/s, φ = 1.2 ± 0.2 rad) had similar stiffness but lower viscosity values than muscle tissue (SWS = 2.9 ± 0.5 m/s, φ = 1.4 ± 0.2 rad), whereas neuroblastoma (SWS = 2.4 ± 0.3 m/s, φ = 0.7 ± 0.1 rad, all p < .05) was the softest and least viscous tissue. Conclusion: Microscopic multifrequency MR elastography-generated maps of zebrafish show many details of viscoelasticity and resolve tissue regions, of great interest in neuromechanical and oncological research and for which our study provides first reference values. |
| Databáze: | OpenAIRE |
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