A fundamental difference in the capacity to induce proliferation of naive T cells between CD28 and other co-stimulatory molecules
| Autor: | Toshiyuki Hamaoka, Michiko Kobayashi, Hiromi Fujiwara, Yumi Yashiro, Ryo Abe, Kazuhito Toyo-oka, Xu-Guang Tai, Steven Neben, Cheung-Seog Park |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
CD3 Complex
T-Lymphocytes T cell Immunology CD2 Antigens Apoptosis chemical and pharmacologic phenomena Biology CD5 Antigens Lymphocyte Activation Tetraspanin 29 Mice CD28 Antigens Co-stimulation Antigens CD medicine Animals Immunology and Allergy Cytotoxic T cell Mice Inbred BALB C Membrane Glycoproteins Integrin beta1 Cell Cycle T-cell receptor CD44 CD28 hemic and immune systems Molecular biology Lymphocyte Function-Associated Antigen-1 Cell biology Hyaluronan Receptors medicine.anatomical_structure biology.protein Interleukin-2 CD5 Signal Transduction |
| Zdroj: | European Journal of Immunology. 28:926-935 |
| ISSN: | 1521-4141 0014-2980 |
| DOI: | 10.1002/(sici)1521-4141(199803)28:03<926::aid-immu926>3.0.co;2-0 |
| Popis: | T cell activation requires two signals: a signal from the TCR and a co-stimulatory signal provided by antigen-presenting cells (APC). In addition to CD28, multiple molecules on the T cell have been described to deliver co-stimulatory signals. Here, we investigated whether there exist quantitative or qualitative differences in the co-stimulatory capacity between CD28 and other molecules. Anti-CD28 monoclonal antibody (mAb) and mAb against CD5, CD9, CD2, CD44 or CD11a all induced activation of naive T cells in the absence of APC when co-immobilized with a submitogenic dose of anti-CD3 mAb. [3H]Thymidine incorporation determined 2 days after co-stimulation was all comparable. In contrast to progressive T cell proliferation induced by CD28 co-stimulation, co-stimulation by other T cell molecules led to a decrease in viable cell recovery along with the induction of apoptosis of once activated T cells. This was associated with a striking difference in IL-2 production; CD28 co-stimulation induced progressively increasing IL-2 production, whereas co-stimulation by other molecules produced limited amounts of IL-2. Addition of recombinant IL-2 to the latter cultures corrected the induction of apoptosis, resulting in levels of cellular proliferation comparable to those observed for CD28 co-stimulation. These results indicate that a fundamental difference exists in the nature of co-stimulation between CD28 and other molecules, which can be evaluated by the levels of IL-2 production, but not simply by [3H]thymidine incorporation. |
| Databáze: | OpenAIRE |
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