Lipopolysaccharides from Microcystis Cyanobacteria-Dominated Water Bloom and from Laboratory Cultures Trigger Human Immune Innate Response
| Autor: | Zdena Moosova, Ondřej Vašíček, Lukáš Kubala, Mirna Velki, Lenka Švihálková Šindlerová, Barbora Ambrůzová, Pavel Babica, Gabriela Ambrožová |
|---|---|
| Jazyk: | chorvatština |
| Rok vydání: | 2019 |
| Předmět: |
Cyanobacteria
congenital hereditary and neonatal diseases and abnormalities endotoxin Lipopolysaccharide Phagocyte leukocytes Health Toxicology and Mutagenesis Population lcsh:Medicine 010501 environmental sciences Toxicology medicine.disease_cause 01 natural sciences cyanobacteria Microbiology 03 medical and health sciences chemistry.chemical_compound Microcystis medicine Microcystis aeruginosa education Axenic Escherichia coli water bloom lipopolysaccharide inflammation 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences education.field_of_study biology Chemistry fungi lcsh:R biology.organism_classification 6. Clean water 3. Good health medicine.anatomical_structure lipids (amino acids peptides and proteins) |
| Zdroj: | Toxins, Vol 11, Iss 4, p 218 (2019) Toxins Volume 11 Issue 4 |
| Popis: | Massive toxic blooms of cyanobacteria represent a major threat to water supplies worldwide. Here, the biological activities of lipopolysaccharide (LPS) isolated from Microcystis aeruginosa, the most prominent cyanobacteria in water bloom, were studied. LPS was isolated from complex environmental water bloom samples dominated by M. aeruginosa, and from laboratory cultures of non-axenic as well as axenic M. aeruginosa strains PCC7806 and HAMBI/UHCC130. Employing human blood-based in vitro tests, the LPS isolated from complex water bloom revealed the priming of both major blood phagocyte population monocytes and polymorphonuclear leukocytes documented by the increased surface expression of CD11b and CD66b. This was accompanied by a water bloom LPS-mediated dose-dependent induction of tumor necrosis factor &alpha interleukin-1&beta and interleukin-6 production. In accordance with its priming effects, water bloom LPS induced significant activation of p38 and ERK1/2 kinases, as well as NF-&kappa B phosphorylation, in isolated polymorphonuclear leukocytes. Interestingly, the pro-inflammatory potential of LPS from the axenic strain of M. aeruginosa was not lower compared to that of LPS isolated from non-axenic strains. In contrast to the biological activity, water bloom LPS revealed almost twice higher pyrogenicity levels compared to Escherichia coli LPS, as analyzed by the PyroGene test. Moreover, LPS from the non-axenic culture exhibited higher endotoxin activity in comparison to LPS from axenic strains. Taking the above findings together, M. aeruginosa LPS can contribute to the health risks associated with contamination by complex water bloom mass. |
| Databáze: | OpenAIRE |
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