Centrifugal disc liquid reciprocation flow considerations for antibody binding to COVID antigen array during microfluidic integration

Autor:	Alexander T. Hwu, Masoud Madadelahi, Rie Nakajima, Ehsan Shamloo, Alexandra Perebikovsky, Horacio Kido, Aarti Jain, Algis Jasinskas, Shawna Prange, Philip Felgner, Marc Madou
Rok vydání:	2022
Předmět:	Immunoassay Microfluidics Biomedical Engineering COVID-19 Humans Biological Assay Bioengineering General Chemistry Microfluidic Analytical Techniques Biochemistry
Zdroj:	Lab on a Chip. 22:2695-2706
ISSN:	1473-0189 1473-0197
Popis:	Heterogeneous immunoassays (HI) are an invaluable tool for biomarker detection and remain an ideal candidate for microfluidic point-of-care diagnostics. However, automating and controlling sustained fluid flow from benchtop to microfluidics for the HI reaction during the extended sample incubation step, remains difficult to implement; this leads to challenges for assay integration and assay result interpretation. To address these issues, we investigated the liquid reciprocation process on a microfluidic centrifugal disc (CD) to generate continuous, bidirectional fluid flow using only a rotating motor. Large volumetric flow rates (μL s
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::3c2f119842731321303babb529ac4a45 https://doi.org/10.1039/d2lc00213b Zobrazit plný text záznamu