Blastocyst-induced changes in the bovine endometrial transcriptome
| Autor: | Daniel J Mathew, Claudia Passaro, Trudee Fair, Patrick Lonergan, José María Sánchez, D. Tutt, John A. Browne, Gry Boe-Hansen |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Embryology Embryonic Development Biology Endometrium Embryo Culture Techniques Andrology Transcriptome 03 medical and health sciences Endocrinology Downregulation and upregulation Pregnancy Gene expression medicine Animals RNA Messenger Blastocyst Obstetrics and Gynecology Embryo Cell Biology ISG15 Coculture Techniques In vitro Up-Regulation 030104 developmental biology medicine.anatomical_structure Reproductive Medicine Culture Media Conditioned embryonic structures Cattle Female Interferons |
| Zdroj: | Reproduction. 156:219-229 |
| ISSN: | 1741-7899 1470-1626 |
| Popis: | The objectives of this study were (i) to determine whether blastocyst-induced responses in endometrial explants were detectable after 6- or 24-h co-culture in vitro; (ii) to test if direct contact is required between embryos and the endometrial surface in order to stimulate endometrial gene expression; (iii) to establish the number of blastocysts required to elicit a detectable endometrial response; (iv) to investigate if upregulation of five interferon-stimulated genes (ISGs) in the endometrium was specific to the blastocyst stage and (v) to test if alterations in endometrial gene expression can be induced by blastocyst-conditioned medium. Exposure of endometrial explants to Day 8 blastocysts in vitro for 6 or 24 h induced the expression of ISGs (MX1, MX2, OAS1, ISG15, RSAD2); expression of IFNAR1, IFNAR2, NFKB1, IL1B, STAT1, LGALS3BP, LGALS9, HPGD, PTGES, ITGB1, AKR1C4, AMD1 and AQP4 was not affected. Culture of explants in the presence of more than five blastocysts was sufficient to induce the effect, with maximum expression of ISGs occurring in the presence of 20 blastocysts. This effect was exclusive to blastocyst stage embryos; oocytes, 2-cell embryos or Day 5 morulae did not alter the relative abundance of any of the transcripts examined. Direct contact between blastocysts and the endometrial surface was not required in order to alter the abundance of these transcripts and blastocyst-conditioned medium alone was sufficient to stimulate a response. Results support the notion that local embryo–maternal interaction may occur as early as Day 8 of pregnancy in cattle. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|