Kaempferol Blocks the Skin Fibroblastic Interleukin 1β Expression and Cytotoxicity Induced by 12-O-tetradecanoylphorbol-13-acetate by Suppressing c-Jun N-terminal Kinase
| Autor: | Tae Hoon Kim, Chang-Hyung Choi, Su-Ji Park, Junghee Sung, Sei-Jung Lee, In Sun Min, Seong-Ryeong Lim, Do-Wan Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Blotting
Western Interleukin-1beta Enzyme-Linked Immunosorbent Assay 12-O-Tetradecanoylphorbol-13-acetate Real-Time Polymerase Chain Reaction Article 12-O-tetradecanoylphorbol 13-acetate Cell Line chemistry.chemical_compound Humans TX341-641 Kaempferols Cytotoxicity normal human dermal fibroblast Skin chemistry.chemical_classification reactive oxygen species Reactive oxygen species Nutrition and Dietetics kaempferol integumentary system Nutrition. Foods and food supply c-jun apoptosis JNK Mitogen-Activated Protein Kinases Interleukin Fibroblasts Molecular biology IκBα chemistry Apoptosis Tetradecanoylphorbol Acetate Dermatologic Agents Kaempferol Food Science |
| Zdroj: | Nutrients Nutrients, Vol 13, Iss 3079, p 3079 (2021) Volume 13 Issue 9 |
| ISSN: | 2072-6643 |
| Popis: | Kaempferol, a bioflavonoid present in fruits and vegetables, has a variety of antioxidant and anti-inflammatory capacities, but the functional role of kaempferol in oxidative skin dermal damage has yet to be well studied. In this study, we examine the role of kaempferol during the inflammation and cell death caused by 12-O-tetradecanoylphorbol-13-acetate (TPA) in normal human dermal fibroblasts (NHDF). TPA (5 μM) significantly induced cytotoxicity of NHDF, where a robust increase in the interleukin (IL)-1β mRNA among the various pro-inflammatory cytokines. The skin fibroblastic cytotoxicity and IL-1β expression induced by TPA were significantly ameliorated by a treatment with 100 nM of kaempferol. Kaempferol blocked the production of the intracellular reactive oxygen species (ROS) responsible for the phosphorylation of c-Jun N-terminal kinase (JNK) induced by TPA. Interestingly, we found that kaempferol inhibited the phosphorylation of nuclear factor-kappa B (NF-κB) and the inhibitor NF-κB (IκBα), which are necessary for the expression of cleaved caspase-3 and the IL-1β secretion in TPA-treated NHDF. These results suggest that kaempferol is a functional agent that blocks the signaling cascade of the skin fibroblastic inflammatory response and cytotoxicity triggered by TPA. |
| Databáze: | OpenAIRE |
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