Behavioral and electroencephalographic evaluation of the anticonvulsive activity of Moringa oleifera leaf non-polar extracts and one metabolite in PTZ-induced seizures
| Autor: | C. L. Martínez-González, Víctor Manuel Magdaleno-Madrigal, Maricela Flores-Carrillo, Gerardo Contreras-Murillo, María Eva González-Trujano, Sara Ibeth Luna-Nophal |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine medicine.medical_treatment Metabolite Drug Evaluation Preclinical Pharmaceutical Science Pharmacology Moringa Mice 03 medical and health sciences chemistry.chemical_compound Epilepsy 0302 clinical medicine Seizures Drug Discovery medicine Animals Hexanes Potency Rats Wistar Active metabolite Moringa oleifera Ethanol Plant Extracts Electroencephalography medicine.disease Plant Leaves 030104 developmental biology Anticonvulsant Ethosuximide Complementary and alternative medicine chemistry Pentylenetetrazole Molecular Medicine Anticonvulsants 030217 neurology & neurosurgery medicine.drug |
| Zdroj: | Phytomedicine. 39:1-9 |
| ISSN: | 0944-7113 |
| Popis: | Backgorund Moringa oleifera Lamarck is a species that has long been used in high demand in folk medicine, including for the treatment of epilepsy. Nevertheless, scientific studies demonstrating its anticonvulsant properties and the nature of the bioactive constituents are lacking. Hypothesis/aim The aim of this study was to evaluate the anticonvulsant activities of the Moringa oleifera leaves in non-polar vs. polar extracts using behavioral and electroencephalographic (EEG) analyses in rodents. Methods First, PTZ (80 mg/kg, i.p.)-induced tonic-clonic seizures were assayed via a dose-response (100, 200 and 300 mg/kg, i.p.) evaluation in mice. Then, a dosage of the extracts (100 or 300 mg/kg) and one metabolite (30 mg/kg, i.p.) was selected to evaluate its effect on PTZ (35 mg/kg, i.p.)-induced EEG paroxystic activities in rats compared to the effects of ethosuximide (reference anticonvulsant drug, 100 mg/kg, i.p.). Latent onset of the first paroxystic spike, first seizure and frequency as well as seizure severity, were determined using Racine's scale. Results Moringa oleifera ethanol and hexane extracts produced a delay in the seizure latency in mice and rats; this effect was improved in the presence of the hexane extract containing the active metabolite hexadecanoic acid. The anticonvulsant effects were corroborated in the spectral analysis by the potency of the EEG due to a reduction in the spike frequency and amplitude, as well as in the duration and severity of the seizures. The effects of the hexane extract resembled those observed in the reference antiepileptic drug ethosuximide. Conclusion Moringa oleifera leaves possess anticonvulsant activities due to the complementary of the non-polar and polar constituents. However, the non-polar constituents appear to exert an important influence via the partial participation of fatty acids, providing evidence of the effects of this plant in epilepsy therapy. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect