Effects of a time varying strong magnetic field on release of cytosolic free Ca2+ from intracellular stores in cultured bovine adrenal chromaffin cells
Autor: | Ki Ho Park, Hisao Yamaguchi, Hitoshi Houchi, Hiroshi Miyamoto, Mami Azuma, Keiko Hosokawa, Mitsuo Kitamura, Hideki Kashimoto, Kazuo Minakuchi, Toshitaka Ikehara, Yohsuke Kinouchi, Kazuo Yoshizaki |
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Rok vydání: | 2002 |
Předmět: |
medicine.medical_specialty
Time Factors Thapsigargin Physiology Cells Chromaffin Cells Biophysics Inositol 1 4 5-Trisphosphate Bradykinin Cell membrane chemistry.chemical_compound Electromagnetic Fields Internal medicine Adrenal Glands medicine Animals Radiology Nuclear Medicine and imaging Inositol Receptor Calcium metabolism Biological Transport General Medicine Inositol trisphosphate receptor Cytosol medicine.anatomical_structure Endocrinology chemistry Calcium Cattle Intracellular Signal Transduction |
Zdroj: | Bioelectromagnetics. 23:505-515 |
ISSN: | 1521-186X 0197-8462 |
Popis: | This study was made to explain the mechanisms for the effects of exposure to a time varying 1.51 T magnetic field on the intracellular Ca(2+) signaling pathway. The exposure inhibited an increase in intracellular Ca(2+) concentration ([Ca(2+)](i)) in bovine chromaffin cells induced by addition of bradykinin (BK) to a Ca(2+) free medium. The exposure did not change BK induced production of inositol 1,4,5-trisphosphate (IP(3)). [Ca(2+)](i) was markedly increased in IP(3) loaded cells, and this increase was inhibited by the magnetic field exposure. A similar increase in [Ca(2+)](i) by other drugs, which stimulated Ca(2+) release from intracellular Ca(2+) stores, was again inhibited by the same exposure. However, transmembrane Ca(2+) fluxes caused in the presence of thapsigargin were not inhibited by the magnetic field exposure in a Ca(2+) containing medium. Inhibition of the BK induced increase in [Ca(2+)](i) by the exposure for 30 min was mostly recovered 1 h after exposure ended. Our results reveal that the magnetic field exposure inhibits Ca(2+) release from intracellular Ca(2+) stores, but that BK bindings to BK receptors of the cell membrane and intracellular inositol IP(3) production are not influenced. |
Databáze: | OpenAIRE |
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