Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application
| Autor: | Kurt Kalcher, Slađana Đurđić, Vesna Stanković, Dalibor M. Stanković, Filip Vlahović, Jelena Mutić, Tanja Ćirković-Veličković, Miloš Ognjanović |
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| Rok vydání: | 2021 |
| Předmět: |
Wide-linear range
02 engineering and technology Amperometric response 01 natural sciences Graphene Nanoplatelets Manganese oxide Materials Chemistry Electrochemistry Enzyme immobilization Analytical applications Electrodes Precise determinations Laccase Renewable Energy Sustainability and the Environment Chemistry 010401 analytical chemistry Screen printed carbon electrode (SPCEs) Electrochemical characteristics 021001 nanoscience & nanotechnology Condensed Matter Physics 0104 chemical sciences Surfaces Coatings and Films Electronic Optical and Magnetic Materials Biosensors Polyphenol Detection limits Graphene 0210 nano-technology Biosensor Accuracy and precision Nuclear chemistry |
| Zdroj: | Journal of The Electrochemical Society Journal of Electrochemical Society |
| ISSN: | 1945-7111 0013-4651 |
| DOI: | 10.1149/1945-7111/abeaf2 |
| Popis: | Based on graphene nanoplatelets capability to build block composites, as well as well-known electrochemical characteristic of the manganese oxide materials, in the present research, a nanocomposite, formed from graphene nanoplatelets (GNP) and manganese(IV)-oxide (MnO2) nanoparticles, has been proposed as a novel and convenient support for enzyme immobilization. Performance of screen printed carbon electrodes (SPCEs) was significantly improved after their modification with GNP@MnO2 (SPCE/GNP@MnO2). The polyphenolic index biosensor was prepared by applying the drop coating technique using laccase and Nafion®. Developed biosensor shows a fast and reliable amperometric response toward caffeic acid, as a model compound, at operating potential of +0.40 V (vs Ag/AgCl), with a wide linear range and detection limit of 1.9 μmol l-1. Developed procedure was successfully applied for the determination of polyphenolic indexes in wine samples. Recovery tests indicate excellent accuracy and precision of the method, concluding that the biosensor can offer a fast, accurate, reliable and precise determination of the polyphenolic index. More importantly, our results suggest a great potential for the application in real samples. © 2021 The Electrochemical Society ("ECS"). Published on behalf of ECS by IOP Publishing Limited. This is the peer-reviewed version of the article: Đurđić, S., Stanković, V., Vlahović, F., Ognjanović, M., Kalcher, K., Ćirković Veličković, T., Mutić, J. J.,& Stanković, D. M. (2021). Laccase Polyphenolic Biosensor Supported on MnO2@GNP Decorated SPCE: Preparation, Characterization, and Analytical Application. Journal of Electrochemical Society, 168(3), 037510. [https://doi.org/10.1149/1945-7111/abeaf2] Published version: [https://cer.ihtm.bg.ac.rs/handle/123456789/4523] |
| Databáze: | OpenAIRE |
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