Sialic acids in the extracellular polymeric substances of seawater-adapted aerobic granular sludge
| Autor: | Mark C.M. van Loosdrecht, Thomas R. Neu, Danny R. de Graaff, Simon Felz, Mario Pronk, Yuemei Lin |
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| Rok vydání: | 2019 |
| Předmět: |
Salinity
Glycan Environmental Engineering 0208 environmental biotechnology Sialic acids 02 engineering and technology 010501 environmental sciences 01 natural sciences chemistry.chemical_compound Spectroscopy Fourier Transform Infrared Neuraminic acid Sialoglycoproteins Monosaccharide Seawater Waste Management and Disposal Glycoproteins 0105 earth and related environmental sciences Water Science and Technology Civil and Structural Engineering chemistry.chemical_classification Sewage biology Extracellular Polymeric Substance Matrix Ecological Modeling Biofilm biology.organism_classification Pollution 020801 environmental engineering Sialic acid Biochemistry chemistry Aerobic granular sludge Galactose biology.protein EPS Bacteria |
| Zdroj: | Water Research, 155 |
| ISSN: | 0043-1354 |
| DOI: | 10.1016/j.watres.2019.02.040 |
| Popis: | Sialic acids have been discovered in the extracellular polymeric substances (EPS) of seawater-adapted aerobic granular sludge (AGS). Sialic acids are a group of monosaccharides with a nine-carbon backbone, commonly found in mammalian cells and pathogenic bacteria, and frequently described to protect EPS molecules and cells from attack by proteases or glycosidases. In order to further understand the role of these compounds in AGS, lectin staining, genome analysis of the dominant bacterial species, and shielding tests were done. Fluorescence lectin bar-coding (FLBC) analysis showed an overlap with protein staining, indicating presence of sialoglycoproteins in the EPS matrix. Genome analysis gives a positive indication for putative production of sialic acids by the dominant bacteria Candidatus Accumulibacter. FT-IR analysis shows upon selective removal of sialic acids a decrease in carbohydrates, extension of the protein side chain, and exposure of penultimate sugars. Enzymatic removal of sialic acids results in the removal of galactose residues from the EPS upon subsequent treatment with β-galactosidase, indicating a linkage between galactose and sialic acid at the terminus of glycan chains. This work indicates the importance of sialic acids in the protection of penultimate sugar residues of glycoproteins in EPS, and provides basis for future research in the composition of EPS from biofilms and granular sludge. |
| Databáze: | OpenAIRE |
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